| 茭白黑粉菌耐热突变体的辐射诱变选育与生长特征研究 |
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| 引用本文： | 石敏.茭白黑粉菌耐热突变体的辐射诱变选育与生长特征研究[J].长江蔬菜,2013(18):95-97. |
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| 作者姓名： | 石敏 |
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| 作者单位： | [1]浙江大学原子核农业科学研究所,杭州310029 [2]浙江大学生命科学学院 ,杭州310029 [3]浙江大学农业与生物技术学院,杭州310029 |
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| 基金项目： | 国家公益性行业（农业）科研专项经费项目（200903017-03）,浙江省科技厅院士基金 |
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| 摘 要： | 来自茭白正常膨大肉质茎的黑粉菌（M—T菌株）经5kGy和10kGy 60Co-γ射线辐照后，筛选获得耐40℃的耐热突变体（H—T菌株）各10个菌株，而来自灰茭的黑粉菌（T菌株）未观察到耐热突变体。对3种菌株在不同温度下的生长特性作了初步分析。研究结果表明，M—T和T菌株均不能在32℃及以上温度生长；15～28℃范围内，H—T菌株的生长速率显著快于T菌株，后者又显著快于M—T菌株；相对于最适温度下的延滞期，显著延长延滞期的临界温度分别为15℃（M-T菌株）、20℃（T菌株）和20℃（H-T菌株）。因此，H-T菌株与M-T菌株的生长特性存在较大差异。
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| 关 键 词： | 茭白黑粉菌 辐照诱变 耐热突变体 生长曲线 |
Selection of Thermo-tolerant Mutants of Ustilago esculenta by Irradiation and Their Growth Characteristics |
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| SHI Min,HUANG Jianzhong,JIANG Qinsu,GUO Deping,ZHANG Jingze,CHEN Ziyuan.Selection of Thermo-tolerant Mutants of Ustilago esculenta by Irradiation and Their Growth Characteristics[J].Journal of Changjiang Vegetables,2013(18):95-97. |
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| Authors: | SHI Min HUANG Jianzhong JIANG Qinsu GUO Deping ZHANG Jingze CHEN Ziyuan |
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| Institution: | l ( 1.Institute of Nuclear-agdcuhural Sciences, Zhejiang University, Hangzhou 310029; 2.College of Life Sciences, Zhejiang University; 3.College of Agriculture and Biotechnology, Zhejiang University ) |
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| Abstract: | Ustilago esculenta mutants （H-T strain）, tolerant of 40~C, were obtained from M-T strain irradiated with 5 kGy and 10 kGy of 60Co-γ ray, but no thermo-tolerant mutant was obtained in T strain from U. eseulenta. And then we studied the growth characteristics of M-T, T and H-T strains at 15-40℃. The results showed that both M-T and T strains were unable to grow while the temperature was above 32℃, and H-T strain grew significantly quicker than T strain at 15-28℃, but T strain grew significantly quicker than M-T strain. The critical temperature significantly prolonged lag phase was 15℃ for M-T strain, 20℃ for T strain and 20℃ for H-T strain respectively, compared to those at optimal temperature. In sum, the growth characteristics of the thermo-tolerant H-T strain had large differences with those of M-T strain. |
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| Keywords: | Ustilago esculenta Irradiation-induced mutation Thermo-tolerant mutant Growth curve |
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