| 猕猴桃品种"皖翠"芽变位点的SCAR标记研究 |
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| 引用本文： | 贾兵,朱立武,余兴,叶振风.猕猴桃品种"皖翠"芽变位点的SCAR标记研究[J].安徽农业大学学报,2007,34(2):251-255. |
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| 作者姓名： | 贾兵 朱立武 余兴 叶振风 |
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| 作者单位： | 安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036 |
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| 基金项目： | 安徽省高校青年教师科研项目
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安徽农业大学校科研和教改项目 |
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| 摘 要： | 对 "海沃德"与"皖翠"RAPD扩增的2条分子量为740 bp、649 bp的特异带,进行了分离、纯化、克隆与测序,根据测序结果设计出4条20 bp的特异引物,进行SCAR-PCR扩增.SCAR-PCR扩增结果显示,分子量为649 bp的特异带在"皖翠"与"海沃德"中都扩增出了条带,说明"皖翠"这一RAPD扩增特异带并不存在;"皖翠"扩增出现了分子量为740 bp的特异带,而"海沃德"未出现此带,证明"海沃德"与"皖翠"存在此变异位点,成功地将"皖翠"变异的RAPD标记转化为SCAR标记,此标记可应用于"皖翠"品种的快速鉴定.
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| 关 键 词： | 皖翠 芽变位点 SCAR标记 |
| 文章编号： | 1672-352X（2007）02-0251-05 |
| 修稿时间： | 2006-12-11 |
Study on Wancui sport site by SCAR marker in Actinidia |
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| JIA Bing,ZHU Li-wu,YU Xing,YE Zheng-feng.Study on Wancui sport site by SCAR marker in Actinidia[J].Journal of Anhui Agricultural University,2007,34(2):251-255. |
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| Authors: | JIA Bing ZHU Li-wu YU Xing YE Zheng-feng |
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| Institution: | The Key Laboratory of Pomology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | Two special bands,with the molecular size of 740 bp and 649 bp between Actinidia deliciosa cv.Hayward and Wancui were separated,purified,cloned and sequenced.Two couple of special primers in 20 bp were designed and synthesized for SCAR-PCR.The result of the SCAR-PCR amplification showed that one of the special bands,the molecular size was 649 bp,did not exist actually.There was a piece of special band with the molecular size of 740 bp in the SCAR-PCR of Wancui,but not in that of Hayward.Therefore the special band with 740 bp had been proved to be true DNA fragment between Wancui and Hayward.RAPD marker of genetic variation in Wancui had been transformed to SCAR marker successfully,it could be used for cultivar rapid identification. |
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| Keywords: | Actinidia deliciosa cv Wancui sport site SCAR marker |
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