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渐渗系IL6的遗传评价和渗入片段的鉴定
引用本文:王为.渐渗系IL6的遗传评价和渗入片段的鉴定[J].棉花学报,2009,21(5):394-398.
作者姓名:王为
作者单位:江苏沿海地区农业科学研究所,盐城,224002
摘    要: 用海岛棉3-79的6号染色体渐渗系(简称IL6)和背景亲本TM-1构建了F2、F2:3群体,在2年的田间重复实验中调查了18个重要农艺性状,作遗传评价。并获得F2群体的分子数据,进行QTL定位和单标记分析。结果表明:亲本IL6中的渐渗片段,组成有3-79的6号染色体,3-79的非6号染色体和大部分的TM-1片段。IL6的产量、品质性状都优于受体亲本TM-1,遗传分析也发现产量、品质性状增效基因位点来自IL6,说明所渗入片段起着关键作用。亲本农艺性状表现和遗传分析结果表明,IL6渗入片段含有总铃数、单株铃数、衣分、果枝数、比强度、麦克隆值等性状的主基因。而QTL定位和单标记分析结果显示,IL6渗入片段含有单株铃数、衣分、果枝数、株高、子指、整齐度等性状的QTLs。

关 键 词:渐渗系IL6  农艺性状  遗传分析  QTL鉴定  
收稿时间:2008-08-25;

Genetic Evaluation of Introgression Line 6 and Identification of the Introgressed Segments
WANG Wei.Genetic Evaluation of Introgression Line 6 and Identification of the Introgressed Segments[J].Cotton Science,2009,21(5):394-398.
Authors:WANG Wei
Institution:Agricultural Science Institute of Coastal Region of Jiangsu;Yancheng;Jiangsu 224002;China
Abstract: A material when a foreign chromosome is replaced into the background parent chromosome is called substitution line. Whereas, a material chromosome segments are replaced, is called introgression line. F2 and F2:3 populations of (TM-1×IL6) were constructed with the background parent TM-1 and introgression line 6(IL6) of chromosome 6 of island cotton 3-79. We investigated 18 important agronomic traits in two years' field experiment and made a genetic evaluation. Besides, molecular data of F2 population were obtained and QTL mapping and single marker analysis were carried out. Segments introgressed into IL6 included chromosome 6, non-chromosome 6 of 3-79 and most TM-1. Yield and quality traits of IL6 parent were more excellent than TM-1. Likewise, a genetic analysis was made and alleles increasing genetic effects from IL6 were found. It means that segments introgressed into IL6 play a crucial part. Agronomic traits and genetic analysis indicated that there were major genes controlling total bolls at full-growing stage, boll numbers per plant, lint percent, fruit branches per plant, fiber strength and the micronaire in the segments. QTL mapping and single marker analysis showed that there were QTLs controlling boll numbers per plant, lint percent, fruit branches per plant, plant height, seed index and uniformity ratio in the segments.
Keywords:IL6  agronomic traits  genetic analysis  QTL mapping  
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