| 尿素-SDS—PAGE测定小分子多肽相对分子质量方法的建立 |
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| 引用本文： | 王丽荣,程福亮,陈雷,谷巍.尿素-SDS—PAGE测定小分子多肽相对分子质量方法的建立[J].饲料博览,2013(9):5-8. |
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| 作者姓名： | 王丽荣 程福亮 陈雷 谷巍 |
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| 作者单位： | 山东宝来利来生物工程股份有限公司,山东泰安271000 |
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| 摘 要： | 试验旨在建立一种简易快速测定小分子多肽相对分子质量的SDS—PAGE电泳方法。通过改进分离胶交联度为6％，丙烯酰胺总浓度为15％，并加入6％的尿素，对所测得的多肽相对分子质量进行线性回归分析。结果表明，得到的标准曲线线性相关系数R2达到0．9938，测得条带的相对分子质量分别为9．2129kD，建立的方法操作简单、耗时短，且小分子多肽成像清晰，是一种具有很高实用价值的测定方法。
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| 关 键 词： | SDS—PAGE 小分子多肽 尿素 相对分子质量 转移因子 |
Development of Urea-SDS-PAGE Method for the Relative Molecular Mass of Small Molecular Peptide |
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| WANG Lirong,CHENG Fuliang,CHEN Lei,GU Wei.Development of Urea-SDS-PAGE Method for the Relative Molecular Mass of Small Molecular Peptide[J].Feed Review,2013(9):5-8. |
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| Authors: | WANG Lirong CHENG Fuliang CHEN Lei GU Wei |
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| Institution: | ( Shandong BaoLai-LeeLai Bioengineering Co., Ltd., Taian 271000, Shandong China) |
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| Abstract: | In order to establish a simple and rapid measuring method SDS-PAGE of the relative molecular mass of small molecular peptide. By improving the separation gel, the crosslinking degree was 6%, total acrylamide concentration was 15%, and 6% of urea was added, the relative molecular mass peptides was obtained by linear re- gression analysis. The Results showed that linear correlation coefficient of the standard curve is 0.993 8, and the rel- ative molecular mass of the target protein bands was measured to be 9.212 9 kD. Conclusions this study established the method of simple operation, short time consuming, and clear imaging, this is a kind of measuring method of the relative molecular mass peptides of small molecular peptide, it has a high practical value. |
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| Keywords: | SDS-PAGE small molecular peptide urea relative molecular mass transfer factor |
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