| 黄牛输卵管上皮细胞的体外分离与培养 |
| |
| 引用本文： | 王益兵,宋宇轩,朱广琴,王建刚,程雪妮,曹斌云.黄牛输卵管上皮细胞的体外分离与培养[J].西北农业学报,2007,16(4):39-41. |
| |
| 作者姓名： | 王益兵 宋宇轩 朱广琴 王建刚 程雪妮 曹斌云 |
| |
| 作者单位： | 西北农林科技大学动物科技学院,陕西杨凌,712100 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 为探讨适用于黄牛输卵管单层上皮细胞的培养方法，采用机械剪取及0．25％胰酶消化的方法分离获得上皮细胞，取对数生长期细胞进行MTT比色实验检测细胞活力和生长速度；台盼蓝排斥试验检测活细胞数。结果表明利用机械剪取及0．25％胰酶消化的方法经细胞形态观察可以分离获得黄牛输卵管上皮单层细胞；台盼蓝排斥实验经计数计算传代的上皮细胞活力在90％以上；经冷冻后再解冻的输卵管上皮细胞活力在75％以上。该研究探索建立了一套较为适宜的黄牛输卵管上皮细胞分离、培养模型。
|
| 关 键 词： | 黄牛 输卵管上皮细胞 培养 |
| 文章编号： | 1004-1389（2007）04-0039-03 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2006-10-312006-12-30 |
Study on Separation and Culture of Cattle Epithelium Cells |
| |
| WANG Yi-bing,SONG Yu-xuan,ZHU Guang-qin,WANG Jian-gang,CHENG Xue-ni and CAO Bin-yun.Study on Separation and Culture of Cattle Epithelium Cells[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(4):39-41. |
| |
| Authors: | WANG Yi-bing SONG Yu-xuan ZHU Guang-qin WANG Jian-gang CHENG Xue-ni and CAO Bin-yun |
| |
| Institution: | Animal Sci-Tech College of Northwest A ZHU Guang-qin, WANG Jian-gang, and CAO Bin-yun F University, Yangling Shaar, xi 712100, China |
| |
| Abstract: | To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0.25% trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by MTT method, the viable count was detected by the rejection experiment of trypanblau. The result indicated that this method can isolated epithelium cells through observe the cell morphous, the cell vigor over 90% and defrosting cells vigor over 75%. Set up a suitable model for separation and culture of cattle epithelium cells. |
| |
| Keywords: | Cattle Epithelium cells Culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
