| 猪细小病毒L株VP2基因片段的克隆及序列分析 |
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| 引用本文： | 王丹娜,柴华,刘洪斌,丁国杰,任德强,戴秀丽,姜力,潘兴广.猪细小病毒L株VP2基因片段的克隆及序列分析[J].中国兽药杂志,2008,42(12). |
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| 作者姓名： | 王丹娜 柴华 刘洪斌 丁国杰 任德强 戴秀丽 姜力 潘兴广 |
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| 作者单位： | 哈药集团生物疫苗有限公司,哈尔滨,150069 |
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| 摘 要： | 对自行分离的PPVL株VP2基因片段进行克隆和测序。序列分析表明:L株VP2基因片段与其他PPV毒株的VP2基因片段的核苷酸同源性均在99.19%以上,氨基酸同源性在98.87%以上。表明L株与国内流行毒株之间同源性极为接近,其中与NADL-2(4973)株的亲缘关系最近(核苷酸同源性为99.81%,氨基酸同源性为99.62%)。在决定毒株组织嗜性的关键氨基酸位点上(378,383及436),L株与NADL-2株相同,据此推测L株的毒性与组织嗜性可能与NADL-2相似。
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| 关 键 词： | 猪细小病毒 L株 VP2基因片段 序列分析 |
Cloning and Sequence Analysis of VP2 Gene Fragment of Porcine Parvovirus L Strain |
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| WANG Dan-na,CHAI Hua,LIU Hong-bin,DING Guo-jie,REN De-qiang,DAI Xiu-li,JIANG Li,PAN Xing-guang.Cloning and Sequence Analysis of VP2 Gene Fragment of Porcine Parvovirus L Strain[J].Chinese Journal of Veterinary Drug,2008,42(12). |
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| Authors: | WANG Dan-na CHAI Hua LIU Hong-bin DING Guo-jie REN De-qiang DAI Xiu-li JIANG Li PAN Xing-guang |
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| Abstract: | The VP2 gene fragment of PPV L strain was cloned and sequenced.It demonstrated over 99.19% nucleotide sequence identity and 98.87% amino acid sequence identity with other PPV strains.Phylogenetic tree analysis of VP2 gene showed the closest relationship between L strain and NADL-2(4973)strain.The same amino acid locus of L strain and NADL-2 strain was on 378,383 and 436,which decided the tissue tropism of PPV,hereby the tissue tropism and virulence of L strain was probably similar with NADL-2 strain. |
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| Keywords: | porcine parvovirus L strain VP2 gene fragment phylogenetic tree analysis |
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