Evaluation of biochemical methods for the diagnosis of the avocado sunblotch viroid in Israel |
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| Authors: | Sara Spiegel Miriam Alper R N Allen |
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| Institution: | 1. Virus Laboratory, ARO, The Volcani Center, Bet Dagan, Israel
2. Agricultural Research Centre, 24800, Wollongbar, New South Wales, Australia
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| Abstract: | The reliability of biochemical diagnostic methods for avocado sunblotch viroid (ASBV) was evaluated for the Israeli avocado propagation program. Polyacry-lamide gel electrophoresis (PAGE) was compared with hybridization toin vitro 32P-labeled cDNA and ASBV-RNA probes. Although hybridization to a cDNA probe was the most sensitive method, not all known infected plants were detected. In the light of these results, the problem of diagnosing ASBV in the Israeli propagation program is discussed. |
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