| 砂梨茎尖和腋芽的离体培养 |
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| 引用本文： | 戴洪义,沈德绪,林伯年.砂梨茎尖和腋芽的离体培养[J].浙江大学学报(农业与生命科学版),1988(4). |
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| 作者姓名： | 戴洪义 沈德绪 林伯年 |
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| 作者单位： | 浙江农业大学园艺系,浙江农业大学园艺系,浙江农业大学园艺系 山东莱阳农学院 |
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| 摘 要： | 四个砂梨品种的茎尖和腋芽离体培养,获得了新梢并诱导生根。发现不同时期和不同树龄的植珠上取材外植体的存活率有明显的差异,而且,不同的基本培养基,不同的细胞分裂素单独或配合使用,不同的碳源对新梢增殖的影响有所不同,不同品种生根所需的条件也不同。生根植株移栽后生长良好。
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| 关 键 词： | 组织培养 试管繁殖 梨 |
Shoot-tip and Axillary Bud Culture of Four Pear Cultivars in vitro |
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| Dai Hongyi Shen Dexu Lin Bonian.Shoot-tip and Axillary Bud Culture of Four Pear Cultivars in vitro[J].Journal of Zhejiang University(Agriculture & Life Sciences),1988(4). |
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| Authors: | Dai Hongyi Shen Dexu Lin Bonian |
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| Abstract: | The procedure of shoot-tip and axillary bud culture in vitro of pear(pyrus pyrifclia Nakai)cultivars Viz."Kosuiw,"Hangqing","Huanghua" and "Qingyun" was described.The surviving rate of explants varied greatly when they were collected at different times or from trees at different ages.The multiplicating rate of shoots varied with basal media,cytokinins and carbon sources.The conditions suitable for shoot rooting of four cultivars were different.Rooted shoots have been transferred to the greenhouse and grew well. |
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| Keywords: | tissue culture micropropagation pear (Pyrus pyrifolia) |
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