| 抗猪红细胞膜抗原非凝集型单克隆抗体的研制及鉴定 |
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| 引用本文： | 王云龙,王国强,闵玉涛,李恒思,陈小科,李玉林.抗猪红细胞膜抗原非凝集型单克隆抗体的研制及鉴定[J].西北农业学报,2009,18(4):29-32. |
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| 作者姓名： | 王云龙 王国强 闵玉涛 李恒思 陈小科 李玉林 |
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| 作者单位： | 王云龙(郑州职业技术学院,河南郑州,450121;河南省生物工程技术研究中心,河南郑州,450001);王国强,闵玉涛,李恒思,陈小科,李玉林(河南省生物工程技术研究中心,河南郑州,450001) |
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| 摘 要： | 以猪红细胞膜抗原免疫BALB/c小鼠,应用杂交瘤技术将免疫小鼠的脾细胞与Sp2/0骨髓瘤细胞融合,通过微孔板凝集试验及玻片凝集试验筛选可稳定分泌非凝集型mAb的杂交瘤细胞株,并用有限稀释法进行克隆.制备出一株可稳定分泌非凝集型抗体的杂交瘤细胞株1C2,其亚型为IgG1类,培养上清和腹水效价分别为1:1024和1:108,没有种属交叉血凝反应,为构建双特异性抗体奠定了基础.
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| 关 键 词： | 猪红细胞 单克隆抗体 作凝集型抗体 |
| 收稿时间： | 2009/3/2 0:00:00 |
| 修稿时间： | 2009/4/10 0:00:00 |
Preparation and Characterization of Non-agglutinating Monoclonal Antibodies against Membrane Antigen of Swine Erythrocytes |
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| WANG Yunlong,WANG Guoqiang,MIN Yutao,LI Hengsi,CHEN Xiaoke and LI Yulin.Preparation and Characterization of Non-agglutinating Monoclonal Antibodies against Membrane Antigen of Swine Erythrocytes[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(4):29-32. |
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| Authors: | WANG Yunlong WANG Guoqiang MIN Yutao LI Hengsi CHEN Xiaoke and LI Yulin |
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| Institution: | Zhengzhou Technical College, Zhengzhou Henan 450121, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China;Henan Bioengineering Research Center, Zhengzhou Henan 450001, China |
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| Abstract: | BALB/c mice were immunized with the membrane antigens of swine erythrocytes.The splenocytes of the immunized mice were fused with Sp2/0 myeloma cells by hybridoma technique.The antibodies to common antigen of swine erythrocytes were screened by micro-quantity plate agglutination test method and then the agglutinating antibodies were weeded out by slide hem-agglutination assay.The hybridoma cells secreting non-agglutinating antibodies were cloned by end dilution method.One hybridoma cell 1C2 was obtained, which secreted non-agglutinating antibody.The mAb 1C2 belonged to IgG1, and without specie scross-agglutination reaction.The titers of cultures suppernatant and ascites of 1C2 were 1:1024 and 1:108 respectively.The mAb 1C2 has high titer, affinity and specificity, which could be used for the preparation of bi-specific antibodies (BsAb). |
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| Keywords: | Swine erythrocyte mAb Non-agglutinating antibody |
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