| 热导系玉米种子贮存蛋白的生物指纹图谱分析 |
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| 引用本文： | 李国良.热导系玉米种子贮存蛋白的生物指纹图谱分析[J].黑龙江农业科学,2005(6):25-27. |
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| 作者姓名： | 李国良 |
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| 作者单位： | 黑龙江省农科院玉米所,哈尔滨,150086 |
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| 摘 要： | 利用聚丙稀酰胺凝胶电泳(SDS-PAGE)对热导系玉米种子的可溶性蛋白进行分析，结果表 明，清蛋白、球蛋白、谷蛋白都显示出丰富的带谱。自交系间谱带数目、带的强弱与分布等特点均可 见较明显的差异，可将各供试自交系互相区分出来；醇溶蛋白谱带均较弱，自交系间差异小，难以把 各供试自交系互相区分出来。尽管种子的可溶蛋白质 PAGE 图谱都可作为玉米自交系鉴定的蛋 白质指纹图谱，相比之下，利用清蛋白 PAGE 图谱较为理想。
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| 关 键 词： | 玉米 聚丙稀酰胺凝胶电泳 指纹图谱 |
| 文章编号： | 1002-2767（2005）06-0025-03 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005年7月14日 |
Finger Print Analysis on Seed Protein of Maize Inbred Line |
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| LI Guo-liang.Finger Print Analysis on Seed Protein of Maize Inbred Line[J].Heilongjiang Agricultural Science,2005(6):25-27. |
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| Authors: | LI Guo-liang |
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| Institution: | Maize Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086 |
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| Abstract: | Electrophoretic analysis was made on the seed soluble protein of 9 maize inbred lines by polyacryamide gel electrophoresis(PAGE)techniques.The results showed that the zeins were both simple and differences among varieties were small.It was,therefore,difficult to distinguish all the varieties studied with the zein patterns,Though,the PAGE of the seed soluble proteins all can be regarded as protein fingerprint for identification of 9 maize varieties,the PAGE patterns of the albumins were,however,abundant and the differences among varieties were obvious in bands number distribution and concentration.On the basis of these characteristics,all the varieties studied could be discriminated from each other.It was suggested that PAGE of seed albumin was useful in variety identification in maize. |
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| Keywords: | maize SDS-PAGE finger print |
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