| 红海榄脱水素基因(RsDHN1)的克隆及表达分析 |
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| 引用本文： | 马宏伟,徐远峰,翟金玲,陈旭峰,黄惜.红海榄脱水素基因(RsDHN1)的克隆及表达分析[J].热带作物学报,2010,31(5):804-808. |
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| 作者姓名： | 马宏伟 徐远峰 翟金玲 陈旭峰 黄惜 |
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| 作者单位： | 海南大学热带生物资源教育部重点实验室,海南大学生物科学技术研究所,海南海口,570228 |
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| 基金项目： | 人力资源与社会保障部2008年度高层次留学人才回国工作资助项目，海南大学2009年度科研项目，海南自然科学基金 |
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| 摘 要： | 采用RT-PCR和RACE技术对已获得的红海榄根部其中一个差异表达基因进行克隆,通过Blast分析和序列比对可知,该基因全长cDNA序列包括146bp的5'端非编码区,43bp的3'端非编码区和一个编码241个氨基酸的开放阅读框,命名为RsDHN1基因。该cDNA编码的蛋白具有植物脱水素蛋白的特征性结构,属于SKn类脱水素蛋白,与其它植物的SKn类脱水素蛋白具有43%～55%的氨基酸序列同源性。半定量RT-PCR分析表明,盐胁迫对RsDHN1基因的表达有明显上调作用。
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| 关 键 词： | 红海榄 脱水素 RACE 耐盐基因 |
Cloning and Characterization of Dehydrin Gene of Rhizophora stylosa(RsDHN1) |
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| Ma Hongwei,Xu Yuanfeng,Zhai Jinling,Chen Xufeng and Xi Huang.Cloning and Characterization of Dehydrin Gene of Rhizophora stylosa(RsDHN1)[J].Chinese Journal of Tropical Crops,2010,31(5):804-808. |
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| Authors: | Ma Hongwei Xu Yuanfeng Zhai Jinling Chen Xufeng and Xi Huang |
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| Abstract: | Rhizophora stylosa is one of the most common species in Hainan mangrove forest. In order to identify salt-tolerant-related genes form Rhizophora stylosa, seedlings of Rhizophora stylosa were planted in fresh water or saline. The differentially expressed ESTs(expressed sequence tag) were detected by macroarry in roots of Rhizophora stylosa. Full-length sequence of the EST was identified by RACE techniques(rapid amplification of cDNA ends). It had 943 bp, which contained a 146 bp 5'-UTR(untranslation region), a 43-bp 3'-UTR and an open reading frame of 726 bp, which encoded a protein of 241 amino acid residues. The deduced protein showed a typical domain of plant dehydrin proteins and contains the conserved S and K segments that belonged to SKn class of plant dehydrin. The gene was termed as RsDHN1. RsDHN1 shared 43%~55% identity with the SKn dehydrins of other plant species. RT-PCR analysis showed that salt treatment significantly upregulated the expression of RsDHN1. |
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| Keywords: | RACE |
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