| 转基因球孢白僵菌发酵产物对野生菌株的增效作用 |
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| 引用本文： | 张永军,金凯,罗志兵,蒋小东,裴炎.转基因球孢白僵菌发酵产物对野生菌株的增效作用[J].植物保护学报,2006,33(3):246-250. |
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| 作者姓名： | 张永军 金凯 罗志兵 蒋小东 裴炎 |
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| 作者单位： | 农业部生物技术与作物品质改良重点开放实验室,重庆市农业生物技术重点实验室,西南大学生物技术研究中心,重庆,400716 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金 |
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| 摘 要： | 研究发现,超量表达类枯草杆菌蛋白酶基因CDEP-1的球孢白僵菌菌株Bb0062-15-4-CDEP-1的发酵产物对野生菌株具有明显的杀桃蚜增效作用。进一步分析增效作用与目的基因表达产物关系的结果显示,加入CDEP-1酶活为44.3±0.6U/μL的发酵产物,可使野生菌株对桃蚜的致死中浓度降低4倍以上,共毒系数(CTC)达404.6。但发酵产物经121℃处理20min,增效作用完全丧失。用饱和硫酸铵沉淀蛋白质分别将透析后的重溶蛋白质和上清液用于增效作用测定,结果证明增效成分主要是蛋白类物质。将梯度稀释的发酵产物与浓度为1×107孢子/mL的野生菌株孢子悬浮液混合进行生物测定,发现各处理的致死中时与CDEP-1的活性呈负相关,相关系数为-0.8946。由此推断,发酵产物中的增效物质主要是目的基因表达产物CDEP-1。
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| 关 键 词： | 类枯草杆菌蛋白酶 球孢白僵菌 发酵产物 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005年8月19日 |
Synergistic effect of fermented solution produced by transgenic Beauveria bassiana increasing the insecticidal efficacy of wild-type strain results from subtilisin-like activity |
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| ZHANG Yong-jun,JIN Kai,LUO Zhi-bing,JIANG Xiao-dong and PEI Yan.Synergistic effect of fermented solution produced by transgenic Beauveria bassiana increasing the insecticidal efficacy of wild-type strain results from subtilisin-like activity[J].Acta Phytophylacica Sinica,2006,33(3):246-250. |
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| Authors: | ZHANG Yong-jun JIN Kai LUO Zhi-bing JIANG Xiao-dong and PEI Yan |
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| Institution: | Key Laboratory of Biotechnology and Crop Quality Improvement of Agriculture Ministry, Chongqing Key Laboratory of Agricultural Biotechnology, Biotechnology Research Center of Southwest University, Chongqing 400716, China;Key Laboratory of Biotechnology and Crop Quality Improvement of Agriculture Ministry, Chongqing Key Laboratory of Agricultural Biotechnology, Biotechnology Research Center of Southwest University, Chongqing 400716, China;Key Laboratory of Biotechnology and Crop Quality Improvement of Agriculture Ministry, Chongqing Key Laboratory of Agricultural Biotechnology, Biotechnology Research Center of Southwest University, Chongqing 400716, China;Key Laboratory of Biotechnology and Crop Quality Improvement of Agriculture Ministry, Chongqing Key Laboratory of Agricultural Biotechnology, Biotechnology Research Center of Southwest University, Chongqing 400716, China;Key Laboratory of Biotechnology and Crop Quality Improvement of Agriculture Ministry, Chongqing Key Laboratory of Agricultural Biotechnology, Biotechnology Research Center of Southwest University, Chongqing 400716, China |
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| Abstract: | |
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| Keywords: | Subtilisin-like protease Beauveria bassiana ferment product |
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