| 薄层扫描法测定栀子中栀子苷的含量 |
| |
| 引用本文： | 彭金年,程庚金生,张付远等.薄层扫描法测定栀子中栀子苷的含量[J].安徽农业科学,2014(1):61-62. |
| |
| 作者姓名： | 彭金年 程庚金生 张付远等 |
| |
| 作者单位： | [1]赣南医学院药学院,江西赣州341000 [2]江西环境工程职业学院,江西赣州341000 |
| |
| 摘 要： | 目的]建立利用薄层扫描法测定栀子中栀子苷含量的方法。方法]样品经超声提取，采用硅胶G高效薄层板条带点样，以氯仿-乙酸乙酯-无水甲醇（3：2：1，V／V／V）为展开剂上行展开，在240nm波长进行扫描检测。结果]在0．240—0．640μg/ml范围内，栀子苷浓度与峰面积呈良好线性关系，相关系数r=0．99954，平均回收率为100．45％，RSD为1．69％。结论]该方法简便、准确、重复性好，可用于赣南野生黄栀子中栀子苷的含量测定。
|
| 关 键 词： | 薄层扫描法 栀子（FRUCTUS GARDENIAE） 栀子苷 |
Determination of Geniposide in FRUCTUS GARDENIAE by Thin Layer Chromatography Scanner |
| |
| Institution: | PENG Jin.nian et al (College of Pharmacy, Gannan Medical University, Ganzhou, Jiangxi 341000) |
| |
| Abstract: | Objective ] A method of thin layer chromatography scanner （TLCS） was established to determine the content of geniposide in FRUCTUS GARDENIAE. Method] The samples were extracted by the method of ultrasonic extraction and the silica gel G thin layer plate was adopted in the determination with chloroform-ethyl acetate-methanol used as developer. The quantitative analysis was conducted by single wavelength scanning at 240 nm. Result] The linear range was 240 -640 ng with correlation coefficient of 0.999 54. The average recovery was 100.45% （RSD = 1.69% ）. Conclusion] The method is simple, accurate, reproducible, and can be used for content determination of geniposide in FRUCTUS GARDENIAE. |
| |
| Keywords: | TLCS FRUCTUS GARDENIAE Geniposide |
| 本文献已被 CNKI 维普 等数据库收录！ |
