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| H2O2溶液对 2种红葡萄离体叶中主要茋化物含量的影响 | |
| 引用本文： | 田春芳,张波,刘景磊,李荣飞,王晓琴,陈韩英.H2O2溶液对 2种红葡萄离体叶中主要茋化物含量的影响[J].中国农学通报,2013,29(6):85-90. |
| 作者姓名： | 田春芳 张波 刘景磊 李荣飞 王晓琴 陈韩英 |
| 作者单位： | 新疆特种植物药资源省部共建教育部重点实验室/石河子大学药学院,新疆石河子,832002 |
| 基金项目： | 国家自然科学基金项目“葡萄茋类物质积累与氧应激反应偶联机制研究”(31160058);兵团青年科技创新资金专项“葡萄白藜芦醇积累的氧应激机制研究”(2010JC47);石河子大学自然科学与技术创新重点项目“氧应激对肿瘤恶性表型调控及新疆地产植物药抗氧化成分干预研究”(ZRKX2009ZD01) |
| 摘 要： | 比较H2O2诱导后美国红提(‘红地球’)和紫香无核(‘新农4号’)离体葡萄叶片中的白藜芦醇(Res)、白藜芦醇苷(PD)、紫檀芪(PS)、ε-二聚体葡萄素(ε-VF)4种芪化物的含量,为利用葡萄生产废弃叶片的综合利用提供依据.采用H2O2溶液对红提和紫香无核离体葡萄叶片进行诱导,采用高效液相色谱法分析4种芪化物的含量变化.H2O2诱导后的两种离体叶片4种芪化物的积累呈现显著的量时依赖性；2.5％H2O2处理48 h后叶片4种芪化物含量可提高6倍以上,以红提的含量相对较高,总芪化物鲜重达到138.82μg/g.用H2O2诱导后的离体葡萄叶中4种芪化物含量比诱导前有了较为明显的增加. |
| 关 键 词： | 茋化物 H2O2诱导 离体葡萄叶 高效液相色谱法 |
| 收稿时间： | 2012/7/31 0:00:00 |
| 修稿时间： | 2012/9/25 0:00:00 |
The Impact of H2O2 Treatment on Stilbene Contents in the Detached Leaves of Two Cultivars Red Grapevine |
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| Tian Chunfang , Zhang Bo , Liu Jinglei , Li Rongfei , Wang Xiaoqin , Chen Hanying.The Impact of H2O2 Treatment on Stilbene Contents in the Detached Leaves of Two Cultivars Red Grapevine[J].Chinese Agricultural Science Bulletin,2013,29(6):85-90. | |
| Authors: | Tian Chunfang Zhang Bo Liu Jinglei Li Rongfei Wang Xiaoqin Chen Hanying |
| Institution: | (Key Laboratory of Xinjiang Phytomedicine Resources of the State Ministry of Education,College of Pharmacology of Shihezi University,Shihezi Xinjiang 832002) |
| Abstract: | The aims were to compare the contents of resveratrol(Res), piceide(PD), pterostilbene (PS), ε-viniferin (ε-VF) in the grape leaves of Hongti (‘Vitis vinifera cv. Red globe’) and Zixiangwuhe (‘Vitis vinifera cv. NO.4 Xin nong’) treated with H2O2, and provide the basis for the comprehensive utilization of waste grape leaves. In present study, the detached grape leaves Hongti and Zixiangwuhe were treated with H2O2 solution to investigate the changes of stilbenes contents by HPLC methods. The results showed those stilbenes accumulated significantly in both dose-dependent and time-dependent manners in H2O2-treated leaves. 4 kinds of stilbenes increased 6 times higher than untreated, with 2.5% H2O2 for 48 hours treatment. Stilbenes contents of Hongti grape leaves was relatively high, the maximum contents of the total stilbenes compounds was 138.82 μg/g in treated leaves. H2O2-treated detached grape leaves showed notably increase on stilbenes contents than Blank. |
| Keywords: | HPLC |
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