| 4个不同世代凡纳滨对虾群体AFLP遗传多样性分析 |
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| 引用本文： | 王豪杰,蔡生力,叶斐菲,刘红.4个不同世代凡纳滨对虾群体AFLP遗传多样性分析[J].上海海洋大学学报,2010(6):739-743. |
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| 作者姓名： | 王豪杰 蔡生力 叶斐菲 刘红 |
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| 作者单位： | 上海海洋大学农业部水产种质资源与养殖生态重点开放实验室, 上海 201306 |
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| 基金项目： | 上海市教育委员会重点学科建设项目(J50701) |
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| 摘 要： | 利用AFLP(amplified fragment length polymorphism)技术对4个不同世代的凡纳滨对虾(Litopenaeus vannamei)群体进行遗传分析,探讨亲本、F1、F2和Fn4个世代群体间的遗传差异。选取10对AFLP引物组合对4个世代凡纳滨对虾群体共80尾个体进行比较分析,共扩增出483个位点,其中348个为多态位点;4个群体的多态位点比例为:61.90%、49.28%、50.52%、46.38%;4个群体的Shannon多样性指数分别为:0.3134、0.2621、0.2594、0.2262;结果表明亲本与F1,F2与Fn相互间遗传距离更为紧密。通过对4个不同世代群体的遗传多样性研究,为下一步进行杂交选育提供理论支持。
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| 关 键 词： | 凡纳滨对虾 AFLP 遗传多样性 世代 |
| 收稿时间： | 2010/4/21 0:00:00 |
AFLP analysis on genetic diversity of four different generations of Litopenaeus vannamei |
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| WANG Hao-jie,CAI Sheng-li,YE Fei-fei,LIU Hong.AFLP analysis on genetic diversity of four different generations of Litopenaeus vannamei[J].Journal of Shanghai Ocean University,2010(6):739-743. |
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| Authors: | WANG Hao-jie CAI Sheng-li YE Fei-fei LIU Hong |
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| Institution: | Key Laboratory of Aquatic Genetic Resources and Aqua-cultural Ecosystem Certified by the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | The AFLP technique was used to analyze the genetic diversity of 4 different generation groups of Litopenaeus vannamei,which were primary parent,F1 generation,F2 generation and Fn generation.10 pair AFLP selective primer scanned 20 samples from each generation and 483 loci were amplified,348 loci were polymorphic loci.The proportions of polymorphic loci among the 4 generation samples were 61.90%, 49.28%, 50.52% and 46.38% respectively.The genetic diversities of the 4 different generations quantified by Shannon index were 0.313 4,0.262 1,0.259 4 and 0.226 2 respectively.The result shows that the genetic distance between primary parent and F1 generation sample is closer than that between F2 and Fn generation samples.The research of genetic diversity among 4 different generations could provide theoretical support for future study on hybrid selective breeding. |
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| Keywords: | Litopenaeus vannamei AFLP genetic diversity different generation groups |
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