| EAsdiA基因的克隆和作为新靶标对梨火疫病菌的检测 |
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| 引用本文： | 高岩,宋俊贤,张蕾,姜英华,胡白石,刘凤权,许志刚.EAsdiA基因的克隆和作为新靶标对梨火疫病菌的检测[J].农业生物技术学报,2007,15(2):312-317. |
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| 作者姓名： | 高岩 宋俊贤 张蕾 姜英华 胡白石 刘凤权 许志刚 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划) |
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| 摘 要： | 基因sdiA属于与群体效应相关的LuxR家族,目前已证实存在于Escherichia coli和Salmonella typhimurium基因组中。本研究从梨火疫病菌(Erwinia amylovora)中克隆到了一个sdiA的同源基因,命名为EAsdiA(GenBank登录号:AY864839),该基因与E.coli和S.typhimurium的sdiA基因在氨基酸水平上分别有45.42%和43.33%的同源性,与其它细菌的luxR同源基因的同源性更低。根据梨火疫病菌EAsdiA和其它病原细菌的luxR基因的序列比对设计了1对特异引物F-EAluxR和R-EAluxR,能够特异地检测梨火疫病菌,检测灵敏度为10个菌体,在含有梨组织浸出液中可检测到102个菌体。本研究首次从梨火疫病菌中克隆sdiA基因并作为新靶标应用于该病菌分子检测。
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| 关 键 词： | 群体感应 梨火疫病菌 分子检测 |
| 文章编号： | 1006-1304（2007）02-0312-06 |
| 收稿时间： | 2006-06-27 |
| 修稿时间： | 2006-09-11 |
EAsdiA Gene Clone and Detection to Erwinia amylovora as a New Target |
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| GAO Yan,SONG Jun-xian,ZHANG Lei,JIANG Ying-hua,HU Bai-shi,LIU Feng-quan,XU Zhi-gang.EAsdiA Gene Clone and Detection to Erwinia amylovora as a New Target[J].Journal of Agricultural Biotechnology,2007,15(2):312-317. |
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| Authors: | GAO Yan SONG Jun-xian ZHANG Lei JIANG Ying-hua HU Bai-shi LIU Feng-quan XU Zhi-gang |
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| Institution: | Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | The gene sdiA, belongs to LuxR family involved in quorum sensing, is present in genomes of Escherichia coli and Salmonella typhimurium. A homologue of sdiA was cloned from Erwinia amylovora and named as EAsdiA (GenBank accession No. AY864839), it shared 45.42% amino acid identity with E. coli and 43.33% with S. typhimurium, respectively. Based on regions of luxR-homologue in E. amylovora where divergent from other luxR-homologues reported, the specific primers F-EAluxR and R-EAluxR were designed for detection of E. amylovora. Ten E. amylovora strains were successfully identified by using these primers from a range of closely related bacteria. Less than 10 cells could be detected from pure culture and as few as 102 bacteria could be confirmed while pear tissue extraction existed. This is the first report of cloning EAsdiA from E. amylovora and as a new target applied for molecular detection of Erwinia amylovora. |
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| Keywords: | LuxR |
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