| 长期有机污水灌溉对土壤固氮细菌种群的影响 |
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| 引用本文： | 张晶,张惠文,苏振成,李新宇,张成刚.长期有机污水灌溉对土壤固氮细菌种群的影响[J].农业环境科学学报,2007,26(2):662-666. |
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| 作者姓名： | 张晶 张惠文 苏振成 李新宇 张成刚 |
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| 作者单位： | 1. 中国科学院沈阳应用生态研究所,辽宁,沈阳,110016;中国科学院研究生院,北京,100039
2. 中国科学院沈阳应用生态研究所,辽宁,沈阳,110016 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 固氮细菌是土壤生态系统中十分重要的功能群之一，在土壤氮素循环中发挥着不可替代的作用。本文通过传统培养方法和固氮基因（nifH）PCR—DGGE指纹图谱分析方法，从微生物功能群角度，研究了长期有机污水灌溉对土壤表层和亚表层固氮细菌种群数量和多样性的影响。结果表明，土壤表层固氮细菌数量随土壤污染的增高而不同程度减少，亚表层自生固氮菌数量无明显变化。从DGGE指纹图谱结果来看，对照清洁土壤的固氮细菌种群多样性较丰富，其Shannon指数在表层为2．4674，在亚表层为2．8210，分别高于受有机污水灌溉不同年限的土壤固氮细菌种群shannon指数，且种群的相似程度有所差异。说明种群结构发生了一定的变化。改清灌3a和改清灌30a土壤固氮细菌种群shannon指数分别略有增加，但种群结构并没有得到完全恢复。污水灌溉对土壤固氮细菌种群的影响具有长期的生态效应，是导致土壤生态与结构功能改变的重要因素之一。
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| 关 键 词： | 污灌 固氮细菌 nifH基因 多样性 |
| 文章编号： | 1672-2043(2007)02-0662-05 |
| 修稿时间： | 2006-10-31 |
The Effect of Long-term Organic Wastewater Irrigation on the Soil Nitrogen-fixing Bacteria Population |
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| ZHANG Jing,ZHANG Hui-wen,SU Zhen-cheng,LI Xin-yu,ZHANG Cheng-gang.The Effect of Long-term Organic Wastewater Irrigation on the Soil Nitrogen-fixing Bacteria Population[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2007,26(2):662-666. |
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| Authors: | ZHANG Jing ZHANG Hui-wen SU Zhen-cheng LI Xin-yu ZHANG Cheng-gang |
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| Institution: | 1.Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | The nitrogen-fixing bacterium is one of the important functional groups in soil ecosystem, and plays a vital role in nitrogen cycling. To study the effect of organic wastewater irrigation on the nitrogen-fixing bacteria population, its population number and nitrogenase gene (nifH) diversity in soil surface and subsurface were analyzed through the traditional culture-dependent method and nifH gene PCR-DGGE fingerprint pattern analysis from the point of microbial functional group. The results showed that the number of nitrogen-fixing bacteria population in soil surface was decreased with the contamination level increasing, while that in soil subsurface had no marked change. This indicated that the wastewater irrigation had a significant influence on the number of the nitrogen-fixing bacteria in surface, while the number of nitrogen-fixing bacteria population in clean soil was not the most among the investigated soil samples. According to the DGGE fingerprint pattern of PCR-amplified nitrogenase gene (nifH) fragments, the shannon index of nitrogen-fixing bacteria population diversity in the clean soil was 2.467 4 in surface and 2.821 0 in the subsurface, being richer than that in polluted soil for nearly 50 years, and in soil irrigated by the clean water for 3 years and after 30 years irrigation with organic wastewater, respectively. The structure of nitrogen-fixing bacteria population in the polluted soil had a modification during the wastewater irrigation. After the clean water irrigation for 3 years or 30 years, the Shannon index of nitrogen-fixing bacteria population diversity increased respectively, but the population structure did not reconvert entirely. From above results, long-time organic wastewater irrigation had long-time ecological effects on nitrogen-fixing bacteria population and its function, and was one of the important factors affecting the soil ecological processes and function. |
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| Keywords: | PCR-DGGE |
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