| 磁珠富集法筛选方正银鲫的三、四核苷酸重复微卫星DNA |
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| 引用本文： | 曹柱,鲁翠云,郑先虎,储志远,张天奇,赵元凤,孙效文.磁珠富集法筛选方正银鲫的三、四核苷酸重复微卫星DNA[J].淡水渔业,2011,41(1):22-28. |
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| 作者姓名： | 曹柱 鲁翠云 郑先虎 储志远 张天奇 赵元凤 孙效文 |
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| 作者单位： | 曹柱,储志远,张天奇,CAO Zhu,CHU Zhi-yuan,ZHANG Tian-qi(大连海洋大学生命科学与技术学院,辽宁大连,116023;中国水产科学研究院黑龙江水产研究所,哈尔滨,150070);鲁翠云,郑先虎,LU Cui-yun,ZHENG Xian-hu(中国水产科学研究院黑龙江水产研究所,哈尔滨,150070;上海海洋大学生命科学与技术学院,上海,200090);赵元凤,ZHAO Yuan-feng(大连海洋大学生命科学与技术学院,辽宁大连,116023);孙效文,SUN Xiao-wen(中国水产科学研究院黑龙江水产研究所,哈尔滨,150070) |
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| 基金项目： | 中国水产科学研究院黑龙江水产研究所基本科研专项 |
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| 摘 要： | 通过生物素磁珠富集法开发方正银鲫(Carassius auratus gibelio (Bloch))三、四核苷酸重复的微卫星,并与传统的放射性同位素杂交法相结合,进行二次筛选微卫星分子标记.结果显示:对652个阳性克隆进行测序分析,获得微卫星序列800个,其中完美型542个(67.75%),非完美型140个(17.5...
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| 关 键 词： | 方正银鲫(Carassius auratus gibelio (Bloch)) 微卫星 磁珠富集法 |
Development of polymorphic tri-and tetranucleotide repeat microsatellite loci in Fangzheng silver crucian carp(Carassius auratus gibelio(Bloch)) by magnetic beads |
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| CAO Zhu,LU Cui-yun,ZHENG Xian-hu,CHU Zhi-yuan,ZHANG Tian-qi,ZHAO Yuan-feng,SUN Xiao-wen.Development of polymorphic tri-and tetranucleotide repeat microsatellite loci in Fangzheng silver crucian carp(Carassius auratus gibelio(Bloch)) by magnetic beads[J].Freshwater Fisheries,2011,41(1):22-28. |
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| Authors: | CAO Zhu LU Cui-yun ZHENG Xian-hu CHU Zhi-yuan ZHANG Tian-qi ZHAO Yuan-feng SUN Xiao-wen |
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| Institution: | CAO Zhu1,2,LU Cui-yun2,3,ZHENG Xian-hu2,CHU Zhi-yuan1,ZHANG Tian-qi1,ZHAO Yuan-feng1,SUN Xiao-wen2 (1.Institute of Life Science and Technology,Dalian Ocean University,Dalian,Liaoning 116023,2.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fisheriy Sciences,Haerbin 150070,3.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306) |
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| Abstract: | Magnetic beads enriched method was used to isolated polymorphic tri-and tetranucleotide repeat microsatellite DNA from Fangzheng silver crucian carp(Carassius auratus gibelio(Bloch)) combining the traditional radioisotope hybridization.As a result,652 positive clones were sequenced after the second selection.800 microsatellite sequences were isolated from these positive clones.There were 542(67.75%) perfect motifs,140(17.50%) imperfect motifs and 118(14.75%) compound in these sequences.163 pairs of primers ... |
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| Keywords: | Carassius auratus gibelio(Bloch) microsatellite magneticbeads enrichment |
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