| 异源Harpin对软腐欧氏杆菌与植物互作关系的影响 |
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| 引用本文： | 张晋丹,李艳琴,申泉,赵立平.异源Harpin对软腐欧氏杆菌与植物互作关系的影响[J].植物病理学报,2001,31(4):330-335,341. |
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| 作者姓名： | 张晋丹 李艳琴 申泉 赵立平 |
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| 作者单位： | 山西大学, 山西省生物工程开放实验室, 太原 030006 |
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| 基金项目： | 国家自然科学基金(39770509);山西省自然科学基金(971065) |
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| 摘 要： | 本文通过三亲交配将带有梨火疫欧文氏杆菌功能完整的hrp基因簇的重组粘粒pCPP430转移到了胡萝卜软腐欧氏杆菌(Erwinia carotovora) Se9R中。从接合子中提纯的质粒与pCPP430的大小、酶切图谱相同;以该质粒为模板,用针对hrpN序列的引物经PCR扩增得到与hrpN大小相同的片段;用地高辛标记的hrpN作为探针进行Southern blotting分析,在接合子中的质粒上得到预期的杂交带。接合子可以在烟草叶片上稳定地引起过敏反应。另外,接合子可以在寄主马铃薯叶片上引起过敏反应样枯斑,表明异源harpin的表达及功能不受软腐菌的抑制。该接合子在体外产生的果胶酸裂解酶活性与受体菌Se9R无明显差别。低浓度的接合子在马铃薯块茎上的软腐症状明显低于受体菌Se9R。外源harpin表达可能不改变软腐欧氏杆菌的致病力、而是通过诱导马铃薯抗性达到减轻发病的作用。
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| 关 键 词： | 胡萝卜软腐欧氏杆菌(Erwinia carotovora) 过敏反应 harpin 致病性 诱导抗性 |
| 文章编号： | 0412-0914(2001)04-0330-06 |
THE INFLUENCE OF IN CELL EXPRESSED HarpinEa ON THE INTERACTION BETWEEN Erwinia carotovora AND HOST AND NON-HOST PIANT |
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| ZHANG Jin dan,LI Yan qin,SHEN Quan,ZHAO Li ping.THE INFLUENCE OF IN CELL EXPRESSED HarpinEa ON THE INTERACTION BETWEEN Erwinia carotovora AND HOST AND NON-HOST PIANT[J].Acta Phytopathologica Sinica,2001,31(4):330-335,341. |
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| Authors: | ZHANG Jin dan LI Yan qin SHEN Quan ZHAO Li ping |
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| Institution: | Shanxi University, Shanxi Key Laboratory of Biotechnology, Taiyuan 030006, China |
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| Abstract: | The recombinant cosmid pCPP430 harboring the functional hrp gene cluster of Erwinia amylovora , which produce an elicitor named harpin Ea was transformed into Erwinia carotovora strain Se9R by tri parental mating. Results of restriction enzymatic mapping, PCR amplification with hrpN specific primers and Southern blotting of plasmid DNA with Dig labeled hrpN DNA as probe showed evidents of the successful construction of the conjugant E.carotovora (pCPP430). The conjugant induced hypersensitive response on non host plant tobacco. It also induced hypersensitive like response on leaves of the host plant potato. The conjugant exhibited reduced virulence on potato tube at low inoculum concentration, but retained the same level of pectate lyase activity as that of the recipient Se9R. It was postulated that the apparent reduced virulence of E.carotovora (pCPP430) may be resulted from improved resistance induced by harpin Ea . |
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| Keywords: | Erwinia carotovora hypersensitive response harpin Ea pathogenicity induced resistance |
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