| 抗冻基因及其在基因工程中的应用研究进展 |
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| 引用本文： | 郝凤,刘晓静,周爱琴,于铁峰.抗冻基因及其在基因工程中的应用研究进展[J].草原与草坪,2009(3):73-78,91. |
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| 作者姓名： | 郝凤 刘晓静 周爱琴 于铁峰 |
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| 作者单位： | 甘肃农业大学,草业学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省农牧厅生物技术专项“抗旱耐盐转基因豆科牧草新品种培育-苜蓿抗寒转基因品种选育”(GNSW-2004-07)资助 |
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| 摘 要： | 低温是限制植物分布与生长的重要因素，低温伤害是一种严重的自然灾害，全球每年因此造成农作物的损失高达数千亿美元。本文综述了抗寒基因研究中一些已分离和鉴定出的低温诱导表达基因，对抗冻基因的功能特性和作用机制进行了全面的回顾，总结了抗冻基因工程的研究方向，对典型抗冻基因的表达效果进行了比较分析，并提出此领域尚存在的一些问题及发展前景。
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| 关 键 词： | 抗冻基因 抗冻机制 基因工程 |
Antifreeze gene and its application in gene engineering |
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| HAO Feng,LIU Xiao-jing,ZHOU Ai-qin,YU Tie-feng.Antifreeze gene and its application in gene engineering[J].Grassland and Turf,2009(3):73-78,91. |
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| Authors: | HAO Feng LIU Xiao-jing ZHOU Ai-qin YU Tie-feng |
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| Institution: | College of Pratacultural Science;Gansu Agricultural University;Lanzhou 730070;China. |
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| Abstract: | Hypothermia is to limit the distribution and growth of plants important factor,Low-temperature injury is a serious natural disasters,The world′s crops each year resulting in the loss of up to hundreds of billions of dollars. Antifreeze genetic engineering is the field of biotechnology has been one of the hot research. Cold resistance gene has attracted wide attention from many countries scholars since it was been discovered. Many crops,especially fruits and vegetables,not only during cultivation in the fields,but also in post-harvest cold storage,frozen storage and frozen will be encountered during the processing of frozen injury. However, using conventional breeding methods to improve the cold tolerance of crops will encounter many difficulties. With the development of molecular biology,gene cloning technology involved in the progressive study of plant cold-hardiness,In this paper,resistance gene has been isolated and identified some of the low-temperature-induced gene expression,function and antifreeze mechanisms of cold resistance gene are reviewed,frost summed up the direction of genetic engineering research,on the typical effects of antifreeze genes in a comparative analysis of expression,and put forward in this field are still some problems and prospects. |
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| Keywords: | cold resistance gene antifreezing mechanism gene engineering |
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