Milk whey as a serum supplement for the growth of FMD virus in BHK-21 cells |
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| Authors: | S N Saha A K Sen |
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| Institution: | (1) Indian Veterinary Research Institute, Bangalore Campus, 560 024 Karnataka, India |
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| Abstract: | Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2×106/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type  A and the virus titres obtained were comparable with those in Eagle's MEM-G with 10% serum. The use of milk whey could reduce the consumption of serum required for the growth of FMD virus in BHK-21 cells by up to 90%. |
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| Keywords: | virology milk serum tissue culture technique foot-and-mouth disease vaccine |
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