| 灰葡萄孢菌nit突变体的诱导及其在营养体亲和性测定中的应用初探 |
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| 引用本文： | 高俊明,刘慧平,王建明,李新凤,韩巨才.灰葡萄孢菌nit突变体的诱导及其在营养体亲和性测定中的应用初探[J].植物病理学报,2003,33(3):233-236. |
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| 作者姓名： | 高俊明 刘慧平 王建明 李新凤 韩巨才 |
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| 作者单位： | 山西农业大学农学院植病系, 太谷 030801 |
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| 基金项目： | 山西省留学基金资助项目 (990 51 ) |
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| 摘 要： | 从山西运城、临汾、长治、晋中、大同等地保护地黄瓜灰霉病病株上采集、分离的分属于3个不同菌丝融合群的8个灰葡萄孢菌单孢菌株,经氯酸盐诱导处理,共获得了抗氯酸盐的硝酸盐利用缺陷突变体(nit突变体)59株,其中nit1型38株,nit3型10株,nitM型11株。所有nit突变株分别在PDA斜面转管培养3次(21 d)后,除6株恢复成野生菌株外,其余多数nit突变菌株表现稳定。来源于同一野生菌株的不同类型nit突变体间或同一菌丝融合群不同野生菌株的nit突变体间可产生互补反应而形成异核体,其中以nitM型突变株互补性最好,在利用nit突变体测定灰葡萄孢菌营养体亲和性时应作为标准菌株。来源于不同菌丝融合群的nit突变体间不能产生互补反应。
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| 关 键 词： | 灰葡萄孢菌 nit突变体 营养体亲和性 异核体 |
| 文章编号： | 0412-0914(2003)03-0233-04 |
| 修稿时间： | 2002年4月11日 |
Induced nitrate non-utilizing mutants of Botrytis cinerea and their use in vegetative compatibility tests |
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| GAO Jun ming,LIU Hui ping,WANG Jian ming,LI Xin feng,HAN Ju cai.Induced nitrate non-utilizing mutants of Botrytis cinerea and their use in vegetative compatibility tests[J].Acta Phytopathologica Sinica,2003,33(3):233-236. |
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| Authors: | GAO Jun ming LIU Hui ping WANG Jian ming LI Xin feng HAN Ju cai |
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| Institution: | Department of Plant Pathology, Agronomy College, Shanxi Agricultural University, Taigu 030801, China |
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| Abstract: | Eight single spore isolates of Botrytis cinerea that belong to three different anastomosis groups were obtained from grey mould cucumber plants collected from Yuncheng, Linfen, Changzhi, Jinzhong, Datong in Shanxi Province. All of the 8 isolates produced chlorate resistant sector on MMC media and nitrate non utilizing mutants ( nit mutant),which were recovered by transferring the chlorate resistant sectors to the MM media containing nitrate as single nitrogen source. Among the 59 nit mutants, 38 nit mutants were nit1 ,10 nit mutants were nit3 ,and 11 nit mutants were nitM . Majority of nit mutants were stable. Complementation occurred between the different nit mutant phenotypes derived from the same parental strain or different strain of the same anastomosis group. Complementation occurred readily and reliably in nitM which was used as the tester to identify the vegetative compatibility group to which isolates B.cinerea belongs. No complementation was observed between any nit mutants recovered from the strains of different anastomosis groups. |
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| Keywords: | Botrytis cinerea nit mutant vegetative compatibility heterokaryon |
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