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盐胁迫对虉草种子萌发与幼苗生理生化的影响
引用本文:丛丽丽,张蕴薇,陈新,李永祥,张新全.盐胁迫对虉草种子萌发与幼苗生理生化的影响[J].草业科学,2011,28(12):2136-2142.
作者姓名:丛丽丽  张蕴薇  陈新  李永祥  张新全
作者单位:1. 四川农业大学草业科学系,四川雅安,625014
2. 中国农业大学动物科技学院草业科学系,北京,100193
3. 新疆维吾尔族自治州草原总站,新疆乌鲁木齐,830049
基金项目:科技支撑2009BADA7B04;“973”课题(2012CB215300);国家现代牧草产业技术体系(CARS-35);北京市重点实验室
摘    要:采用0(CK)、50、100、150、200和250 mmol/L 6个NaCl浓度梯度,对6份虉草(Phalaris arundinacea)材料的种子萌发和幼苗生长2个阶段进行盐胁迫处理。通过对种子的发芽率、鲜质量、苗高、根长、脯氨酸含量、丙二醛和叶绿素含量等生理化指标的测定,观测其在盐胁迫下的一系列生理生化反应。结果表明,低NaCl浓度对种子萌发及幼苗生长这2个阶段的影响较小,随着NaCl浓度的增高,种子发芽率、根长、鲜质量、苗长、叶绿素含量逐渐降低,脯氨酸含量显著增加,丙二醛含量呈先降低后增加的趋势。6份虉草材料在种子发芽和幼苗生理变化上均表现出不同的耐盐能力,Zxy06p 2449、Zxy06p 1904耐盐能力较强,其次为Zxy06p 2380、Zxy06p 2633,Zxy06p 2262和Zxy06p 1916的耐盐性较差。

关 键 词:NaCl胁迫  虉草  种子萌发  脯氨酸  丙二醛  叶绿素

Effect of NaCl stress on seed germination and physiological and biochemical characteristics of seedlings of Phalaris arundinacea
CONG Li-li,ZHANG Yun-wei,CHEN Xin,LI Yong-xiang,ZHANG Xin-quan.Effect of NaCl stress on seed germination and physiological and biochemical characteristics of seedlings of Phalaris arundinacea[J].Pratacultural Science,2011,28(12):2136-2142.
Authors:CONG Li-li  ZHANG Yun-wei  CHEN Xin  LI Yong-xiang  ZHANG Xin-quan
Institution:1(1.Department of Grassland Science,Animal Science and Technology College, Sichuan Agricultural University,Sichuan Ya’an 625014,China; 2.Institute of Grassland Science,China Agricultural University,Beijing 100193,China; 3.Grassland General Station of Xinjiang,Xinjiang Urumqi 830049,China)
Abstract:An experiment was conducted to determine the effect of NaCl stress on seed germination and physiological and biochemical characteristics of seedlings of 6 accessions Phalaris arundinacea by measuring seed germination rate,fresh weight,shoot length,root length,proline content,chlorophyll and malonaldehyde(MDA) content.The NaCl stress consisted of 0(CK),50,100,150,200 and 250 mmol/L.The results of this study showed that lower NaCl concentration did not significantly affected seed germination and seedling growth of P.arundinacea;however,the germination rate,fresh weight,shoot length,root length and chlorophyll content of P.arundinacea decreased,and the proline content significantly increased,and MDA content decreased firstly decreased and then increased as the NaCl concentration increased.This study indicated that 6 accessions of P.arundinacea had the different salt tolerance in seed germination and physiological property of seedling,in which Zxy06p-2449 and Zxy06p-1904 were the strongest salt tolerant,and Zxy06p-2380 and Zxy06p-2633 were intermediate,and Zxy06p-2262 and Zxy06p-1916 were sensitive to salt stress.
Keywords:NaCl stress  Phalaris arundinacea  germination  proline  MDA  chlorophyll
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