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| 不同游离方式辣椒小孢子的胚胎发生 | |
| 引用本文： | 成妍,巫东堂,马蓉丽,魏学红,焦彦生,吴海涛.不同游离方式辣椒小孢子的胚胎发生[J].山西农业科学,2012,40(7):705-708. |
| 作者姓名： | 成妍 巫东堂 马蓉丽 魏学红 焦彦生 吴海涛 |
| 作者单位： | 1. 山西大学生命科学学院,山西太原030006;山西省农业科学院蔬菜研究所,山西太原030032 2. 山西省农业科学院蔬菜研究所,山西太原,030032 3. 山西大学化工学院,山西太原,030006 |
| 基金项目： | 山西省农业科学院博士基金 |
| 摘 要： | 以甜椒、牛角椒、羊角椒、线椒和朝天椒5个不同类型的10个辣椒品种为试材,研究通过直接机械游离、看护培养自然游离和先花药预培养再机械游离3种不同的小孢子游离方式的胚胎发生情况。结果表明,先花药预培养再机械游离小孢子的胚胎发生最好,看护培养自然游离小孢子的胚胎发生次之,直接机械游离小孢子的胚胎发生最差,仅获得少量球形胚;小孢子在培养前期需要花药壁的存在,高温预处理之后则需要花药壁及时破裂。 |
| 关 键 词： | 辣椒 小孢子 游离方式 胚胎发生 胚状体 |
Embryogenesis of Pepper Microspore through Diferent Isolation Methods |
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| CHENG Yan , WU Dong-tang , MA Rong-li , WEI Xue-hong , JIAO Yan-sheng , WU Hai-tao.Embryogenesis of Pepper Microspore through Diferent Isolation Methods[J].Journal of Shanxi Agricultural Sciences,2012,40(7):705-708. | |
| Authors: | CHENG Yan WU Dong-tang MA Rong-li WEI Xue-hong JIAO Yan-sheng WU Hai-tao |
| Institution: | 1.College of Life Science,Shanxi University,Taiyuan 030006,China;2.Institute of Vegetables,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China;3.College of Chemical Engineering,Shanxi University,Taiyuan 030006,China) |
| Abstract: | Embryogenesis of microspores through three different isolation methods,including direct mechanical isolating,shed-culture nature isolating,and mechanical isolating after anther pre-culture,were studied by using 10 Capsicum varieties belonging to 5 different fruit types of pepper as the materials.The results showed that embryogenesis of microspores through mechanical isolating after anther pre-culture was the best,then shed-culture nature isolating,and direct mechanical isolating was the worst,which got only some global embryoids;anther wall was necessary to microspores at the beginning of culture,and must be ruptured in time after high temperature pre-treatment. |
| Keywords: | pepper microspore isolating method embryogenesis embryoid |
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