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| 醇沉条件对猴头菌多糖得率和品质的影响 | |
| 引用本文： | 蒋俊,罗珍,刘艳芳,彭川丛,吴迪,周帅,张劲松,冯志勇,杨焱.醇沉条件对猴头菌多糖得率和品质的影响[J].食用菌学报,2011,18(2):57-61. |
| 作者姓名： | 蒋俊 罗珍 刘艳芳 彭川丛 吴迪 周帅 张劲松 冯志勇 杨焱 |
| 作者单位： | 1. 无限极中国有限公司,广东广州510665;农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403;南京农业大学生命科学学院,江苏南京210095 2. 无限极中国有限公司,广东广州,510665 3. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403 |
| 基金项目： | 上海市农委重点攻关项目[编号:沪农科攻字(2008)第8-2号]的部分研究内容 |
| 摘 要： | 以粗多糖得率和含量为评价指标,对水提醇沉制备猴头菌多糖(Hericium erinaceuspolysaccharides,HEP)工艺中主要醇沉条件进行了筛选,得出猴头菌多糖醇沉的最佳工艺条件:提取液的浓缩比(粗提液体积∶子实体干重,mL∶g)为3∶1,醇沉浓度70%,加入乙醇时浓缩液温度为40℃,醇沉时间和静置温度分别为8 h和4℃。不同醇沉浓度得到的多糖分子量分布及体外免疫活性测定结果表明,醇沉浓度为40%时,得到的多糖大分子部分占的比例较高,活性较好。醇沉浓度越高,多糖含有的小分子物质越多。 |
| 关 键 词： | 醇沉工艺 浓缩比 醇沉浓度 分子量分布 免疫活性 |
Effect of Precipitation Conditions on the Yield and Quality of Hericium erinaceus Polysaccharides |
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| JIANG Jun,LUO Zhen,LIU Yanfang,PENG Chuancong,WU Di,ZHOU Shuai,ZHANG Jingsong,FENG Zhiyong,YANG Yan.Effect of Precipitation Conditions on the Yield and Quality of Hericium erinaceus Polysaccharides[J].Acta Edulis Fungi,2011,18(2):57-61. | |
| Authors: | JIANG Jun LUO Zhen LIU Yanfang PENG Chuancong WU Di ZHOU Shuai ZHANG Jingsong FENG Zhiyong YANG Yan |
| Institution: | JIANG Jun1,2,3,LUO Zhen1,LIU Yanfang2,PENG Chuancong1,WU Di2,ZHOU Shuai2,ZHANG Jingsong2,FENG Zhiyong2,YANG Yan2 1Infinitus Company Ltd,Guangzhou,Guangdong 510000,China,2National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,3College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095 |
| Abstract: | The conditions used for ethanol precipitation of Hericium erinaceus polysaccharides from aqueous extracts of mushroom fruit bodies were optimized based on yield and polysaccharide content.Higher yields and polysaccharide contents were obtained under the following conditions: concentration ratio(extract volume∶dry weight of fruit body),3∶1(mL∶g);ethanol concentration used for precipitation,70%;initial temperature of concentrated extract,40 ℃;precipitation time and temperature,8 h and 4 ℃ respectively.Molecular weight distribution determinations revealed that the proportion of macromolecular material was high in polysaccharide precipitated with 40% ethanol,while increasing ethanol concentrations precipitated more material of lower molecular weight.Polysaccharide precipitated with 40% ethanol also exhibited higher immunological activity. |
| Keywords: | Ethanol precipitation concentration ratio ethanol concentration immunological competence |
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