In vitro effects of DDT analogs on trout brain Mg2+-ATPases: I. Specificity and physiological significance |
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| Authors: | DA Jackson DR Gardner |
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| Institution: | Department of Biology, Carleton University, Ottawa, Ontario, Canada K1S 5B6 |
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| Abstract: | A continuous steady-state assay procedure was used to investigate the effects of DDT and several analogs on the in vitro Mg2+-stimulated adenosinetriphosphatase of a trout brain mitochondrial fraction. Pharmacological dissection of the enzyme with oligomycin, dicyclohexyl-carbodiimide, and azide failed to yield a fraction specifically sensitive to the organochlorines. At 25°C, low doses of DDT (≤1.35 μmol/mg of protein) stimulated enzyme activity, while methoxychlor was stimulatory at all doses. Higher doses of DDT and of several analogs caused only 45.5% or less inhibition at 25°C, but inhibition increased at lower temperatures. The physiological significance of these effects is discussed. |
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| Keywords: | To whom reprint requests should be sent |
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