In vitro effects of DDT analogs on trout brain Mg2+-ATPases: Inhibition kinetics |
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| Authors: | DA Jackson DR Gardner |
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| Institution: | Department of Biology, Carleton University, Ottawa, Ontario, Canada K1S 5B6 |
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| Abstract: | A continuous in vitro steady-state assay procedure was used to investigate the dependence of trout brain mitochondrial Mg2+-stimulated adenosinetriphosphatase specific activity on temperature and substrate concentration. The inhibition of enzyme activity by DDT was independent of substrate concentration. DDT and several analogs caused increases in the experimental activation energy and frequency factor of the enzyme-catalyzed reaction, which gave rise to a negative temperature coefficient of inhibition. It is suggested that DDT and other highly lipophilic compounds have the potential to allosterically affect membrane-bound enzymes by simply becoming a major lipoid component of the membranes. |
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| Keywords: | To whom reprint requests should be sent |
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