| 番茄褪绿病毒侵染黄瓜的首次报道 |
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| 引用本文： | 王天旗,史晓斌,郑立敏,刘 勇,张德咏,谭新球,周序国.番茄褪绿病毒侵染黄瓜的首次报道[J].植物保护,2020,46(2):91-95. |
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| 作者姓名： | 王天旗 史晓斌 郑立敏 刘 勇 张德咏 谭新球 周序国 |
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| 作者单位： | 1. 湖南大学研究生院隆平分院, 长沙 410125; 2. 湖南省农业科学院植物保护研究所, 长沙 410125 |
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| 基金项目： | 国家自然科学基金（31872932，31672003）; 湖南省科技计划（2016RS2019）; 国家大宗蔬菜产业技术体系(CARS-25-B-05) |
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| 摘 要： | 番茄褪绿病毒Tomato chlorosis virus(ToCV)是严重危害世界经济作物的一种病毒,寄主范围广泛。田间调查发现黄瓜Cucumis sativus表现出叶片黄化、脉间褪绿的疑似番茄褪绿病毒感病症状,同时叶片背面聚集了大量烟粉虱。采用RT-PCR方法对样品叶片和烟粉虱进行检测,ToCV感染率为65%,且发病叶片上烟粉虱携带ToCV。为进一步确定黄瓜是否为番茄褪绿病毒的新寄主,室内利用农杆菌侵染性克隆接种健康黄瓜,结果显示:接种30 d的黄瓜新生叶片出现褪绿症状。采用ToCV HSP70基因的引物对田间黄瓜叶片、烟粉虱和室内黄瓜新生叶片进行RT-PCR,扩增出约450 bp的条带,在NCBI上BLAST显示与KC887999.1的同源性最高,为99%。这些数据表明黄瓜是番茄褪绿病毒的寄主。这是ToCV感染黄瓜的首次报道。
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| 关 键 词： | 番茄褪绿病毒 黄瓜 烟粉虱 RT-PCR |
| 收稿时间： | 2019/3/4 0:00:00 |
| 修稿时间： | 2019/4/7 0:00:00 |
Cucumber (Cucumis sativus) is a new host for Tomato chlorosis virus |
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| WANG Tianqi,SHI Xiaobin,ZHENG Limin,LIU Yong,ZHANG Deyong,TAN Xinqiu,ZHOU Xuguo.Cucumber (Cucumis sativus) is a new host for Tomato chlorosis virus[J].Plant Protection,2020,46(2):91-95. |
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| Authors: | WANG Tianqi SHI Xiaobin ZHENG Limin LIU Yong ZHANG Deyong TAN Xinqiu ZHOU Xuguo |
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| Institution: | 1. Long Ping Branch,Graduate School of Hunan University, Changsha 410125, China; 2. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China |
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| Abstract: | Tomato chlorosis virus (ToCV) causes severe damage to crops worldwide, and it infects a wide range of plant hosts. In a field investigation, we found leaf etiolation, interveinal chlorosis of suspected symptoms of tomato chlorosis virus disease in cucumber (Cucumis sativus); meanwhile a large number of Bemisia tabaci gathered on the back of cucumber leaves. The leaves and B.tabaci were tested by RT-PCR. The results showed that the ToCV infection rate was 65%, and B.tabaci on the infected leaves carried ToCV. In order to further determine whether cucumber is the new host of tomato chlorosis virus, healthy cucumbers were inoculated with agrobacterium-infected clones indoors. The results showed that chlorosis appeared in new leaves of cucumbers 30 days after inoculation. The primers of ToCV HSP70 gene were used to perform RT-PCR on cucumber leaves, whiteflies and new leaves of indoor cucumber, and a band of 450 bp was amplified. BLAST on NCBI showed the highest homology (99%) with KC887999.1. These data indicate that cucumber is the host of tomato chlorosis virus. This is the first report of ToCV infection in cucumbers. |
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| Keywords: | Tomato chlorosis virus Cucumis sativus Bemisia tabaci RT-PCR |
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