| 营养液Cu2＋、Zn2＋和Mn2＋浓度对低温胁迫下黄瓜幼苗叶片SOD活性的影响 |
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| 引用本文： | 李涛,于贤昌.营养液Cu2＋、Zn2＋和Mn2＋浓度对低温胁迫下黄瓜幼苗叶片SOD活性的影响[J].中国农业科学,2008,41(3):772-778. |
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| 作者姓名： | 李涛 于贤昌 |
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| 作者单位： | 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金
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山东省自然科学基金 |
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| 摘 要： | 【目的】探讨Cu2＋、Zn2＋和Mn2＋在黄瓜幼苗抗冷中的作用。【方法】以自根和嫁接黄瓜幼苗为试材，研究了用含有不同（Cu2＋+Zn2＋+Mn2＋）整体浓度营养液育苗对幼苗叶片Cu、Zn和Mn含量、低温胁迫下超氧化物歧化酶(SOD)及其同工酶活性和电解质渗漏率的影响。【结果】提高营养液（Cu2＋+Zn2＋+Mn2＋）整体浓度，自根黄瓜幼苗叶片Cu、Zn和Mn含量显著增加，低温胁迫下其SOD、Mn-SOD及Cu/Zn-SOD活性均显著增强，且低温胁迫时间越长活性增强越显著，Fe-SOD活性和电解质渗漏率则显著降低；降低营养液（Cu2＋+Zn2＋+Mn2＋）整体浓度培育嫁接黄瓜幼苗的结果则与之相反。【结论】Cu、Zn和Mn在黄瓜幼苗抗冷性中具有重要作用。以黑籽南瓜为砧木嫁接的黄瓜，根系吸收Cu2＋、Zn2＋和Mn2＋能力较强是其抗冷性增强的内在因素之一；自根黄瓜幼苗叶片Cu、Zn和Mn含量和SOD活性因营养液（Cu2＋+Zn2＋+Mn2＋）整体浓度升高而升高，从而增强其抗冷性。
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| 关 键 词： | 嫁接黄瓜 自根黄瓜 微量元素 低温胁迫 SOD 电解质渗漏率 |
| 收稿时间： | 2006-12-01 |
| 修稿时间： | 2007-03-27 |
Effects of Different Concentrations of Cu2+, Zn2+ and Mn2+ in Nutrient Solution on Leaf SOD Activity of Grafted and Own-Root Cucumber Seedlings Under Low Temperature Stress |
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| LI Tao,YU Xian-chang.Effects of Different Concentrations of Cu2+, Zn2+ and Mn2+ in Nutrient Solution on Leaf SOD Activity of Grafted and Own-Root Cucumber Seedlings Under Low Temperature Stress[J].Scientia Agricultura Sinica,2008,41(3):772-778. |
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| Authors: | LI Tao YU Xian-chang |
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| Abstract: | 【Objective】The aim of this study is to investigate the functions of Cu2＋, Zn2＋and Mn2 on cold tolerance of cucumber seedlings. 【Method】The contents of Cu, Zn and Mn and the changes of Superoxide Dismutase(SOD) and its isoenzymes activities and electrolytic leakages of own-root and grafted cucumber seedlings leaves cultivated by different concentrations of Cu2＋, Zn2＋and Mn2＋under low temperature were studied. 【Result】The contents of Cu, Zn and Mn ,the activities of SOD, Mn-SOD and Cu/Zn-SOD of own-root cucumber seedling leaves cultivated by high concentrations of Cu2＋, Zn2＋and Mn2＋ in nutrient solution were higher than the control, while the Fe-SOD activity and electrolytic leakages were lower; When grafted cucumber seedlings were cultivated by low concentrations of Cu2＋, Zn2＋and Mn2＋in nutrient solution, the results were contrary.【Conclusion】The functions of Cu2＋, Zn2＋and Mn2 on cold tolerance of cucumber seedlings were very important. And it was proved that the roots of grafted cucumber seedlings which could absorb more Cu, Zn and Mn after grafted by figleaf gourd was one of the reasons that the grafted cucumber seedlings could have more chilling tolerance than own-root cucumber seedlings. The contents of Cu, Zn and Mn and SOD activity of own-root cucumber seedlings both could be enhanced by increasing the contents of Cu2＋,Zn2＋and Mn2＋in nutrient solution, so that the chilling tolerance also could been enhanced. |
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| Keywords: | Grafted cucumber Own-root cucumber Microelement Low temperature stress SOD Electrolytic leakage |
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