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Photomorphogenic mutants of tomato
Authors:R E Kendrick  L H J Kerckhoffs  A S Pundsnes  A Van Tuinen  M Koorneef  A Nagatani  M J Terry  A Tretyn  M -M Cordonnier-Pratt  B Hauser  L H Pratt
Institution:(1) Department of Biochemistry and Genetics, University of Newcastle upon Tyne, NE2 4HH Newcastle upon Tyne, UK
Abstract:Summary Two cloned cassava genes (pCAS6, pHNL) and a barley gene (pBLT63) which is highly conserved in higher plants, were evaluated as potential multilocus probes for RFLP analysis of phylogenetic relationships of species within the genus Manihot. For M. rubricaulis, M. chlorosticta and M. esculenta subsp. flabellifolia dendrograms based on 13 probe-restriction enzyme combinations show very little variation between accessions of the same species. A close relationship is demonstrated between the Mexican species M. chlorosticta and the M. esculenta subsp. flabellifolia accessions, which does not support the classification of flabellifolia as a true South American wild species.
Keywords:cassava  Manihot  RFLP  phylogenetic relationships  multilocus probes
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