| 新批准的西南地区5个山羊遗传资源mtDNA D-loop遗传多样性与亲缘关系研究 |
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| 引用本文： | 赵中权,刘小艳,张旭刚,李周权,赵永聚,张家骅.新批准的西南地区5个山羊遗传资源mtDNA D-loop遗传多样性与亲缘关系研究[J].黑龙江畜牧兽医,2011(19). |
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| 作者姓名： | 赵中权 刘小艳 张旭刚 李周权 赵永聚 张家骅 |
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| 作者单位： | 西南大学重庆市牧草与草食家畜重点实验室; |
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| 基金项目： | 重庆市科委重庆攻关项目(CSTC2,008AA1033); 中央高校基本科研业务费专项资金项目(XDJK2010C095) |
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| 摘 要： | 为了研究新批准的西南地区5个山羊遗传资源的遗传多样性及其与其他羊种的亲缘关系,试验利用设计的引物对山羊的mtDNA D-loop序列进行了PCR扩增和测序,对序列数据进行了核苷酸多样性、单倍型和亲缘关系分析。结果表明:在西南地区新批准的5个山羊遗传资源中,大足黑山羊、黔北麻羊和酉州乌羊平均多态位点数比较接近,均高于渝东黑山羊和贵州黑山羊;大足黑山羊、黔北麻羊和酉州乌羊遗传多样性高于贵州黑山羊和渝东黑山羊;从亲缘关系来看,渝东黑山羊与板角山羊和南江黄羊亲缘关系较近,酉州乌羊与板角山羊亲缘关系最近,贵州3个羊种(贵州白山羊、贵州黑山羊和黔北麻羊)遗传距离近,大足黑山羊与乐至黑山羊遗传距离较近。说明渝东黑山羊和贵州黑山羊遗传多样性较低,需要加强遗传资源的保护和提纯;新批准的5个山羊遗传资源的遗传距离和亲缘关系与其所处的地理位置存在密切关系。
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| 关 键 词： | 山羊遗传资源 西南地区 mtDNA D-loop 遗传多样性 亲缘关系 |
Studies on genetic diversity and sibship of mitochondrial DNA D-loop sequences in five newly approved goat genetic resources in southwest of China |
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| ZHAO Zhong-quan,LIU Xiao-yan,ZHANG Xu-gang,LI Zhou-quan,ZHAO Yong-ju,ZHANG Jia-hua.Studies on genetic diversity and sibship of mitochondrial DNA D-loop sequences in five newly approved goat genetic resources in southwest of China[J].Heilongjiang Animal Science And veterinary Medicine,2011(19). |
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| Authors: | ZHAO Zhong-quan LIU Xiao-yan ZHANG Xu-gang LI Zhou-quan ZHAO Yong-ju ZHANG Jia-hua |
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| Institution: | ZHAO Zhong-quan,LIU Xiao-yan,ZHANG Xu-gang,LI Zhou-quan,ZHAO Yong-ju,ZHANG Jia-hua(Chongqing Key Laboratory of Forage & Herbivore,College of Animal Science and Technology,Southwest University,Chongqing 400715,China) |
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| Abstract: | To explore the genetic diversity and sibship of five newly approved goat genetic resources in southwest of China,the mitochondrial DNA D-loop sequence were amplified and sequenced.The nucleotide diversity,haplotype and genetic relationship analysis were carried out.The results showed that in the five new goat genetic resources,the average number of polymorphic sites in Dazu black goat,Qianbei ma goat and Youzhou wu goat were very near and were higher than the number of polymorphic sites in Yudong black goat... |
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| Keywords: | goat genetic resource southwest mitochondrial DNA D-loop genetic diversity sibship |
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