| 6种番茄叶表皮制片方法比较 |
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| 引用本文： | 宋艳波,杨云浩,段鹏军,王嘉浩,魏丝晴,张亚龙.6种番茄叶表皮制片方法比较[J].农学学报,2022,12(1):45-52. |
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| 作者姓名： | 宋艳波 杨云浩 段鹏军 王嘉浩 魏丝晴 张亚龙 |
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| 作者单位： | 1.山西农业大学生命科学学院,山西太谷 030801;2.山西农业大学信息科学与工程学院,山西太谷 030801 |
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| 基金项目： | 国家自然科学基金“高压脉冲电场协同效应失活封闭型畜禽舍空气中病原体机制研究”(31772651) |
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| 摘 要： | 结合番茄叶片结构特点,采用6种表皮制片技术对番茄叶片下表皮进行装片,并对不同方法的制片过程和观察效果进行比较研究.结果表明,番茄叶片薄,表皮为单层细胞,其上着生有丰富表皮毛,下表皮与疏松的海绵组织相邻;表皮撕取法和透明胶带粘取法可以真实地观测气孔大小、密度等静态性状指标和气孔开度等动态行为;因番茄叶柔软,刮片法对操作要...
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| 关 键 词： | 番茄 表皮制片 气孔 显微观察 方法比较与改良 |
| 收稿时间： | 2021-05-17 |
Comparison Study of 6 Kinds of Tomato Leaf Epidermis Slice Techniques |
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| SONG Yanbo,YANG Yunhao,DUAN Pengjun,WANG Jiahao,WEI Siqing,ZHANG Yalong.Comparison Study of 6 Kinds of Tomato Leaf Epidermis Slice Techniques[J].Journal of Agriculture,2022,12(1):45-52. |
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| Authors: | SONG Yanbo YANG Yunhao DUAN Pengjun WANG Jiahao WEI Siqing ZHANG Yalong |
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| Institution: | 1.College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China;2.College of Information Science and Engineering, Shanxi Agricultural University, Taigu 030801, Shanxi, China |
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| Abstract: | This paper adopted six kinds of leaf epidermis slice techniques to treat lower epidermis of tomato leaves considering their structural characteristics, and compared the preparation process and observation effect of different techniques. The results show that tomato leaves are thin, the epidermis is single-layer cell with abundant epidermal hairs, and the lower epidermis is adjacent to the loose spongy tissue. Static characteristics, such as dynamic behavior of stomatal size, density and stomatal aperture, could be observed by skin peeling method and cellophane tape sticking method. Due to the softness of tomato leaves, the scraping method is extremely demanding to operate, and dissociation method is time-consuming and has poor effect, both the techniques are not suitable for the slice production of tomato leaf epidermis. Tearable nail oil blotting method could obtain the imprint of stomatal index and stomatal aperture, while the leaves would be damaged certainly. Silica gel-nail polish two-stage blotting method has no damage to the leaves, the same part of the same leaf could be imprinted continuously without scathe, and in this way, the dynamic behavior like stomatal movement and stomatal development could be observed continuously. The results could provide a basis for selecting suitable tomato leaf epidermis preparation methods for different research purposes. |
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| Keywords: | Tomato Leaf Epidermis Slice Techniques Stoma Microscopic Observation Method Comparison and Improvement |
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