| 市售农残速测试剂盒对5种有机磷农药的敏感性评价 |
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| 引用本文： | 罗俊霞,赵建波,赵倩倩,张刚,王峰,刘青,张洁,潘玲,赵利敏,马蕾.市售农残速测试剂盒对5种有机磷农药的敏感性评价[J].农学学报,2022,12(8):73-80. |
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| 作者姓名： | 罗俊霞 赵建波 赵倩倩 张刚 王峰 刘青 张洁 潘玲 赵利敏 马蕾 |
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| 作者单位： | 1.郑州市农产品质量检测流通中心,郑州 450006;2.郑州市农业技术推广中心,郑州 450002;3.焦作市农产品质量安全检测中心,河南焦作 454002;4.河南恒晟检测技术有限公司,河南新郑 451100;5.郑州市农业综合行政执法支队,郑州 450002 |
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| 基金项目： | 河南省郑州市农产品质量检测中心建设项目(豫发改农经（2011）1778号);郑州市检测检验体系建设项目(郑农检（2012）12号);郑州市农产品质量安全追溯体系建设项目(郑发改农经[2013]126号) |
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| 摘 要： | 为了对商品化农残速测试剂盒进行评价,全面了解市售农残速测试剂盒的质量,本研究采用市售的6个品牌农残速测试剂盒,在2种情况下（提取液取用2.5 mL和3 mL）对甲拌磷、蝇毒磷、二嗪磷、对硫磷、久效磷进行敏感性测定,根据5种农药在5个浓度水平下的测定结果,绘制6个品牌的试剂盒的抑制率对农药浓度的效应曲线并用抑制率和农药浓度之对数进行线性拟合、计算出相关系数、IC50和IC10,根据IC10对各种试剂盒对每种农药的敏感性进行比较并以此判定试剂盒的优劣。结果表明同一种试剂盒对不同的农药、不同的试剂盒对同一种农药的敏感性均不同;抑制率和农药浓度对数相关性较好的试剂盒其相关系数R2分别为0.4835~1（提取液取用2.5 mL）和0.6602~1（提取液取用3 mL）,IC10分别为2.01×10-4~3.78 mg/kg（提取液取用2.5 mL）和3.92×10-3~3.36 mg/kg（提取液取用3 mL）;6种试剂盒对甲拌磷均不敏感,除了A试剂盒外其他试剂盒在5个浓度水平下抑制率均为0。根据所测试的5种农药,将试剂盒分为3个等次,D试剂盒最优,其次是C和E,第三是A、B和F。建议国标和行标修改50%或者70%的判定阈值。
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| 关 键 词： | 试剂盒 农药 半抑制浓度 检出限 敏感性 线性范围 |
| 收稿时间： | 2021-07-08 |
Sensitivity Evaluation of the Test Kits for the Determination of the Residual Rate of Five Organophosphorus Pesticides in Agricultural Products |
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| LUO Junxia,ZHAO Jianbo,ZHAO Qianqian,ZHANG Gang,WANG Feng,LIU Qing,ZHANG Jie,PAN Ling,ZHAO Limin,MA Lei.Sensitivity Evaluation of the Test Kits for the Determination of the Residual Rate of Five Organophosphorus Pesticides in Agricultural Products[J].Journal of Agriculture,2022,12(8):73-80. |
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| Authors: | LUO Junxia ZHAO Jianbo ZHAO Qianqian ZHANG Gang WANG Feng LIU Qing ZHANG Jie PAN Ling ZHAO Limin MA Lei |
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| Institution: | 1.Zhengzhou Agricultural Product Quality Testing and Distribution Center, Zhengzhou 450006, Henan, China;2.;2.Zhengzhou Agricultural Technology Extension Centre, Zhengzhou 450002, Henan, China;3.Jiaozuo Agricultural Products Quality and Safety Testing Center, Jiaozuo 454002, Henan, China;4.Henan Hengsheng Testing Technology Co., Ltd., Xinzheng 451100, Henan, China;5.Zhengzhou Agricultural Comprehensive Administrative Law Enforcement Detachment, Zhengzhou 450002, Henan, China |
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| Abstract: | To evaluate the commercial test kits for pesticide residue rate and understand their quality, the sensitivity of six brands of pesticide residue rapid test kits sold in market to phorate, phoxim, diazinon, parathion and moncrotophos were determined in two cases (2.5 mL and 3 mL of extract). The effect curves of inhibition rate on pesticide concentration of the kits were plotted according to the determination results of the five pesticides at five concentration levels, the logarithm of inhibition rate and pesticide concentration were used for linear fitting, and the correlation coefficient, IC50 and IC10 were calculated, the sensitivity of various kits to each pesticide were compared according to IC10. Then the advantages and disadvantages of the kits were determined. The sensitivity of the same kit to different pesticides and different kits to the same pesticide were different. The kits with better logarithmic correlation between inhibition rate and pesticide concentration had correlation coefficient R2 of 0.4835-1 (2.5 mL of extract) and 0.6602-1 (3 mL of extract); and IC10 was 2.01×10-4-3.78 mg/kg (2.5 mL of extract) and 3.92×10-3-3.36 mg/kg (3 mL of extract) respectively. The six kits were not sensitive to phorate. Except kit A, the inhibition rates of the other kits at five concentration levels were 0. According to the five pesticides tested, the kits are divided into three grades. D kit is the best, followed by C and E, and the third is A, B and F. It is suggested to modify the judgment threshold of 50% or 70% of the national standard and industrial standard. |
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| Keywords: | test kit pesticide semi-inhibition concentration detection limit sensitivity linear range |
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