Unigene derived SSR analysis for the <Emphasis Type="Italic">Fugu rubripes</Emphasis> and insights into the characteristics of EST-SSR distribution in tissues/organs |
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| Authors: | Jiaqing Wang Lin Hou |
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| Institution: | 1.College of Ocean,Agricultural University of Hebei,Qinhuangdao,China;2.College of Life Sciences,Liaoning Normal University,Dalian,China |
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| Abstract: | A total of 11348 Unigene derived ESTs from five cDNA libraries corresponding to five different tissues/organs were screened
for EST-SSR, and 1097 EST-SSRs (9.67%) were detected. There were considerable variations in the amount of EST-SSRs among the
five tissues/organs. In all the tissues/organs, trinucleotide repeats (67.74%) were found to be most abundant. The trinucleotide
repeats GCG were the most abundant in three tissues/organs that ranged from 80.2% in skin, 83.3% in gut, to 90.2% in fin.
Dinucleotide was the second abundant repeat type (13.58%), followed by tetra-(12.38%), hexa-(5.34%), and penta-(0.96%) nucleotide
repeats. Furthermore, the results of the present study also indicated that the EST-SSR motifs were not randomly distributed
in tissues/organs of fugu, such as trinucleotide repeats were found to be strikingly abundant in fin, followed by skin and
gut, tetra-nucleotide repeats were especially rich in muscle, while pentanucleotide repeats were less common in any tissue
or organ. Most of the EST-SSRs in five tissues/organs that were assigned functions during the present study represent housekeeping
genes. |
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