| ICR小鼠胚胎干细胞的分离与培养 |
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| 引用本文： | 崔雯,熊浩,王杏龙.ICR小鼠胚胎干细胞的分离与培养[J].黑龙江畜牧兽医,2011(3). |
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| 作者姓名： | 崔雯 熊浩 王杏龙 |
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| 作者单位： | 扬州大学动物科学与技术学院; |
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| 基金项目： | “863”国家高技术研究发展计划项目(2008AA101005) |
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| 摘 要： | 为了研究不同条件对ICR小鼠ES细胞的影响,试验以12.5~13.5 d ICR小鼠胚胎成纤维细胞(MEF)为饲养层,以3.5 d ICR小鼠胚胎为试验材料,探讨了血清、生长因子及传代方法对ICR小鼠ES细胞分离培养的影响。结果表明:采用含15%FBS的ES细胞培养液的囊胚贴壁率及ICM增殖率(79.3%,69.0%)均比含15%KSR、5%FBS+10%KSR的细胞培养液高(42.9%,28.6%;75.0%,54.2%),ES细胞最高传至6代;培养液中添加10 ng/mL LIF+10 ng/mL SCF的效果比单独添加1种因子的效果好,最高传至6代,高于单独添加1种因子的传代数(4代,2代);用3种传代方法进行传代时,采用差异贴壁法传代效果最佳,最高传至8代,酶消化法传至4代,机械加酶消化法传至6代。
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| 关 键 词： | ICR小鼠 胚胎干细胞 分离与培养 |
Isolation and cultivation of ICR mouse embryonic stem cells(ESCs) |
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| CUI Wen,XIONG Hao,WANG Xing-long.Isolation and cultivation of ICR mouse embryonic stem cells(ESCs)[J].Heilongjiang Animal Science And veterinary Medicine,2011(3). |
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| Authors: | CUI Wen XIONG Hao WANG Xing-long |
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| Institution: | CUI Wen,XIONG Hao,WANG Xing-long(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | To study the different conditions on ICR mouse ESCs,the mouse embryonic fibroblasts(MEFs) were made by 12.5-to 13.5-day mouse fetus and 3.5-day mouse embryos.The results suggested that the adherence rate of blastocysts and proliferation rate of ICM for the medium contained with 15% FBS(79.3%,69.0%)were both larger than the mediums contained with 15%KSR or 5%FBS ad 10%KSR(42.9%,28.6%;75.0%,54.2%),and the ESC clones were cultured for six passages.The medium added 10 ng/mL LIF and 10 ng/mL SCF was better than ... |
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| Keywords: | ICR mouse embryonic stem cells(ESCs) isolation and cultivation |
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