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| 抗双链RNA依赖性蛋白激酶PKR基因表达载体构建及在玉米中的遗传转化 | |
| 引用本文： | 尹祥佳,翁建峰,谢传晓,郝转芳,李新海,张世煌,李永生,王汉宁.抗双链RNA依赖性蛋白激酶PKR基因表达载体构建及在玉米中的遗传转化[J].华北农学报,2011,26(4). |
| 作者姓名： | 尹祥佳 翁建峰 谢传晓 郝转芳 李新海 张世煌 李永生 王汉宁 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃兰州730070;中国农业科学院作物科学研究所,北京100081 2. 中国农业科学院作物科学研究所,北京,100081 3. 甘肃农业大学农学院,甘肃兰州,730070 |
| 基金项目： | 高产优质粮饲兼用玉米新品种富农1号示范种植(0704XCNA007); 玉米小麦遗传转化新技术(200908010-008B) |
| 摘 要： | 玉米病毒性病害和杂草严重影响其产量和品质。以pCAMBIA5300为基础载体,应用In-Fusion克隆技术构建了双价植物表达载体pCAMBIA5300-Ubi-PKR-CaMV35S-EPSPS,其中含有抗双链RNA依赖性蛋白激酶PKR基因和磷酸烯醇式丙酮酸莽草酸-3-磷酸合酶EPSPS基因,分别由玉米泛素Ubi启动子和花椰菜花叶病毒35 S启动子启动。以玉米种子黄化苗的茎尖分生组织为受体,用农杆菌介导法进行遗传转化,将抗双链RNA依赖性蛋白激酶基因PKR和抗除草剂草甘膦基因EPSPS导入玉米自交系掖478中,获得转基因植株及其子代。 |
| 关 键 词： | 玉米 植物表达载体 农杆菌介导 PKR基因 |
Constructions of Expression Vectors for Double-stranded RNA-dependent Protein Kinase PKR Gene and the Genetic Transformation in Maize |
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| YIN Xiang-jia,WENG Jian-feng,XIE Chuan-xiao,HAO Zhuan-fang,LI Xin-hai,ZHANG Shi-huang,LI Yong-sheng,WANG Han-ning.Constructions of Expression Vectors for Double-stranded RNA-dependent Protein Kinase PKR Gene and the Genetic Transformation in Maize[J].Acta Agriculturae Boreali-Sinica,2011,26(4). | |
| Authors: | YIN Xiang-jia WENG Jian-feng XIE Chuan-xiao HAO Zhuan-fang LI Xin-hai ZHANG Shi-huang LI Yong-sheng WANG Han-ning |
| Institution: | YIN Xiang-jia1,2,WENG Jian-feng2,XIE Chuan-xiao2,HAO Zhuan-fang2,LI Xin-hai2,ZHANG Shi-huang2,LI Yong-sheng1,WANG Han-ning1(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2.Institute of Crop Science,Chinese Academy of Agriculture Sciences,Beijing 100081,China) |
| Abstract: | Corn viral diseases and weeds are serious affecting the yield and quality.pCAMBIA5300 as a based vector had been constructed the plant expression vector pCAMBIA5300-Ubi-PKR-CaMV35S-EPSPS by In-Fusion cloning technique,which contains anti-double-stranded double-stranded RNA-dependent protein kinase PKR gene and phosphoenolpyruvate shikimic acid-3-phosphate synthase EPSPS gene,driven by maize ubi promoter and by cauliflower mosaic virus 35 S(CaMV35S) promoter respectively.The receptors of maize seedlings shoo... |
| Keywords: | Maize(Zea mays L ) Plant expression vector Agrobacterium tumefaciens mediated PKR gene |
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