| 陆地棉Dirigent-like基因(GhDIR)的克隆与分析 |
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| 引用本文： | 赵付安,房卫平,杨晓杰,谢德意,李武,唐中杰.陆地棉Dirigent-like基因(GhDIR)的克隆与分析[J].华北农学报,2011(5):29-33. |
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| 作者姓名： | 赵付安 房卫平 杨晓杰 谢德意 李武 唐中杰 |
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| 作者单位： | 河南省农业科学院经济作物研究所;河南大学生命科学学院; |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项(2008ZX08005-001) |
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| 摘 要： | Dirigent-like基因(DIR)编码蛋白质参与木质素的形成,与植物体的病菌防御有关.本研究以抑制差减杂交( SSH)技术获得DIR的EST为信息探针,对棉花EST数据库进行同源搜索和序列拼接,获得了全长为768 bp的DIR基因cDNA全长,将该基因命名为GhDIR.该基因序列的开放阅读框(ORF)位于70 ～...
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| 关 键 词： | 陆地棉 电子克隆 DIR-like gene 表达分析 大丽轮枝菌 |
Cloning and Analysis of Upland Cotton(Gossypium hirsutum) Dirigent-like Gene(GhDIR) |
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| ZHAO Fu-an,FANG Wei-ping,YANG Xiao-jie,XIE De-yi,LI Wu,TANG Zhong-jie.Cloning and Analysis of Upland Cotton(Gossypium hirsutum) Dirigent-like Gene(GhDIR)[J].Acta Agriculturae Boreali-Sinica,2011(5):29-33. |
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| Authors: | ZHAO Fu-an FANG Wei-ping YANG Xiao-jie XIE De-yi LI Wu TANG Zhong-jie |
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| Institution: | ZHAO Fu-an1,2,FANG Wei-ping1,YANG Xiao-jie1,XIE De-yi1,LI Wu1,TANG Zhong-jie1(1.Institute of Cash Crops Research,Henan Academy of Agricultural Science,Zhengzhou 450002,China,2.College of Life Science,Henan University,Kaifeng 475100,China) |
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| Abstract: | Dirigent-like gene encodes a protein invloved in the formation of lignin,and in defence to pathogen disease.Using an EST of a dirigent-like gene as the query probe to blast cotton EST database,a full-length 768 bp cDNA sequence of the dirigent-like gene was obtained and cloned by PCR,was named GhDIR.Sequence analysis indicated that it contained a complete open reading fragment(ORF),from 70 to 594 bp,encoding 175 amino acids,and has no intron.Sequence comparison and phylogenetic analysis showed the GhDIR cDN... |
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| Keywords: | Gossypium hirsutum In silica cloning DIR-like gene Expression analysis Vertivillium dahlige |
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