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| 硫酸锌慢性毒性胁迫下克氏原螯虾的组织病理 | |
| 引用本文： | 王权,王建国,陆宏达,熊良伟,安健.硫酸锌慢性毒性胁迫下克氏原螯虾的组织病理[J].中国水产科学,2012,19(1):126-137. |
| 作者姓名： | 王权 王建国 陆宏达 熊良伟 安健 |
| 作者单位： | 1. 江苏畜牧兽医职业技术学院 江苏 泰州 225300 2. 江苏畜牧兽医职业技术学院 江苏 泰州 225300;上海海洋大学 水产与生命学院 上海 201306 3. 上海海洋大学 水产与生命学院 上海 201306 4. 连云港市海洋与水产科学研究所 江苏 连云港 222044 |
| 基金项目： | 上海市重点学科建设项目(Y1101);上海海洋大学研究生基金项目(A-2501-09-0000-87047) |
| 摘 要： | 将克氏原螯虾(Procambarus clarkii)成虾在Zn2+质量浓度为0、0.40 mg/L、4.04 mg/L和40.37 mg/L的硫酸锌溶液中浸浴21 d,分别于第7、14和21天时每组随机抽取5只虾,采集肝胰腺、鳃、触角腺、性腺、肌肉及消化道组织,于Bouin′s液中固定24 h。采用石蜡切片和HE染色的方法,观察其组织的病理变化,从组织学水平研究硫酸锌对克氏原螯虾的慢性毒性作用。结果显示,浸浴时各Zn2+浓度组肝胰腺、触角腺细胞质中出现被染成红棕色的颗粒,且颗粒的数量与浸浴浓度及时间成正相关。0.40 mg/L组在浸浴期间各组织均未出现其他明显病变;4.04mg/L组及40.37mg/L组从浸浴第7天开始肝胰腺、触角腺、鳃组织出现出血、细胞坏死等病变,随浸浴浓度增大和时间延长坏死细胞比例增大。40.37 mg/L组第14天时精母细胞开始出现坏死;第21天时精巢、卵巢严重出血,精、卵细胞出现大面积坏死,肝细胞大量坏死脱落,肝小管结缔组织增生,大量脱落的细胞塞满管腔使肝胰腺表面形成肉眼可见的红棕色颗粒状斑点。结果表明在Zn2+质量浓度高于4.04 mg/L的硫酸锌浸浴21 d后,克氏原螯虾肝胰腺、触角腺、鳃、性腺组织均出现不同程度的病变,表明其代谢系统、呼吸系统、生殖系统等受到较严重的损伤,因此长期暴露在Zn2+质量浓度高于4.04 mg/L的环境中会严重威胁克氏原螯虾的生存和繁殖。 |
| 关 键 词： | 克氏原螯虾 硫酸锌 胁迫 组织病理 慢性毒性 |
| 修稿时间： | 2012/2/2 0:00:00 |
Chronic toxicity of zinc sulphate in Procambarus clarkii |
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| WANG Quan,WANG Jianguo,LU Hongd,XIONG Liangwei,AN Jian.Chronic toxicity of zinc sulphate in Procambarus clarkii[J].Journal of Fishery Sciences of China,2012,19(1):126-137. | |
| Authors: | WANG Quan WANG Jianguo LU Hongd XIONG Liangwei AN Jian |
| Institution: | 1.Jiangsu Animal Husbandry & Veterinary College,Taizhou 225300,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China; 3.Marine Fisheries Research Institute of Lianyungang,Lianyungang,222042,China |
| Abstract: | We evaluated the chronic toxicity of zinc sulphate in the crayfish Procambarus clarkii.Mature P.clarkii were exposed to 0,0.40,4.04,or 40.37 mg/L Zn2+ for 21 days.Each day,we randomly selected five crayfish in each group and collected samples of the hepatopancreas,gill,and muscle tissue from males and females and ovar-ian tissue from each female.The tissues were placed in Bouin’s fixative for 24 h,sectioned in paraffin,and stained using H?E.We observed a number of brick red particles in the cytoplasm of cells from the hepatopancreas and antennal gland in animals exposed to Zn2+.The number of these particles was positively correlated with the con-centration of Zn2+ and exposure time.There was no obvious histopathology in the group exposed to 0.40 mg/L Zn2+.We observed bleeding and necrosis in hepatopancreas,antennal gland,and gills in the group exposed to 4.04 mg/L after 7 d.The proportion of necrotic cells increased as the concentration of Zn2+ and time of exposure in-creased.We observed spermatocyte necrosis after 14 d in exposure to 40.37 mg/L Zn2+.After 21 d,we observed bleeding of the spermary and ovary and necrosis of the ootid and spermatocyte cells.A large number of hepato-cytes exhibited necrosis and had dropped from the hepatic ducts.The connective tissue exhibited hyperplasia,with large numbers of cells blocking the hepatic ducts,resulting in macroscopical bulky grains in the hapatopancreas of the group exposed to 40.37 mg/L.Our results suggest that exposure to 4.04 mg/L Zn2+ for 21 d causes pathological changes in the hepatopancreas,antennal gland,gills,and gonad tissue of P.clarkii.Given that zinc sulphate inter-feres with the metabolic,respiratory,and reproductive systems,chronic exposure is likely to threaten the survival of P.clarkii populations. |
| Keywords: | Procambarus clarkii zinc sulphate stress histopathology chronic toxicity |
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