| 培养用液对小鼠胚胎移植效果的影响 |
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| 引用本文： | 李文清,刘全军,赵卫东,李万利.培养用液对小鼠胚胎移植效果的影响[J].河南农业大学学报,2006,40(4):383-385. |
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| 作者姓名： | 李文清 刘全军 赵卫东 李万利 |
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| 作者单位： | 1. 河南农业大学,河南,郑州,450002
2. 华南农业大学,广东,广州,510642 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 为提高小鼠胚胎移植的成功率,探索了合适的胚胎培养用液,研究了原核移植和囊胚移植对产仔率的影响.结果表明,CZB培养液适合ICR小鼠原核胚胎培养,囊胚发育率达到90.67%,囊胚移植的产仔率为53.33%,高于原核移植的34%.受体和供体不必同期处理,前者可比后者晚1 d处理.
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| 关 键 词： | 胚胎移植 小鼠 受体 供体 |
| 文章编号： | 1000-2340（2006）04-0383-03 |
| 收稿时间： | 2005-10-28 |
| 修稿时间： | 2005年10月28 |
Effects of Culture Medium on Embryo Transfer in Mice |
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| LI Wen-qing,LIU Quan-jun,ZHAO Wei-dong,LI Wan-li.Effects of Culture Medium on Embryo Transfer in Mice[J].Journal of Henan Agricultural University,2006,40(4):383-385. |
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| Authors: | LI Wen-qing LIU Quan-jun ZHAO Wei-dong LI Wan-li |
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| Institution: | 1. Henan Agricultural University, Zhengzhou 450002, China ; 2. South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | To improve the success rate of embryo transfer in mice,the suitable culture medium was explored and its effects on rate of full-term young of one-cell transfer and blastula transfer were studied.The results showed that CZB medium was fit for the culture of one-cell embryo of ICR mice and the rate of embryos developed to blastula was 90.67%.The rate of transferred embryos developed to term in blastula stage((53.33%)) was higher than that in one-cell stage (34%).Simultaneous treatment wasn't mandatory and recipient might be disposed one day later than the donor. |
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| Keywords: | embryo transfer mice recipient donor |
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