| 黄色马蹄莲多倍体诱导研究 |
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| 引用本文： | 邵果园,梁国鲁.黄色马蹄莲多倍体诱导研究[J].浙江林学院学报,2008,25(5):630-634. |
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| 作者姓名： | 邵果园 梁国鲁 |
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| 作者单位： | 1. 浙江林学院,农业与食品科学学院,浙江,临安,311300
2. 西南大学,园艺园林学院,重庆,400716 |
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| 基金项目： | 浙江林学院校科研和教改项目 |
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| 摘 要： | 为探明秋水仙素诱导彩色马蹄莲多倍体的最佳处理方法．以黄色马蹄莲奇妙Zantedeschia elliottian‘Black Magic’球茎为材料，在离体条件下诱导愈合组织并产生芽点．放入0．2，0．5及1．0g·L^-1秋水仙素溶液中分别浸泡24，48和72h，诱导黄色马蹄莲多倍体。结果显示：秋水仙素能成功诱导黄色马蹄莲多倍体．其最佳处理是使用0．5g·L^-1秋水仙素浸泡芽点48h．四倍体诱导率13．3％。四倍体植株体细胞染色体数目为2n=4x=64。获得的四倍体植株与二倍体比较，外部形态特征、气孔特性等方面有显著性差异，具有植株强健，叶片变大增厚，保卫细胞增大，叶绿体数目增多．气孔密度减少等特点．图3表3参18
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| 关 键 词： | 园艺学 黄色马蹄莲 组织培养 秋水仙素 多倍体 |
Polyploidy induction in Zantedeschia elliottiana |
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| SHAO Guo-yuan,LIANG Guo-lu.Polyploidy induction in Zantedeschia elliottiana[J].Journal of Zhejiang Forestry College,2008,25(5):630-634. |
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| Authors: | SHAO Guo-yuan LIANG Guo-lu |
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| Institution: | SHAO Guo-yuan, LIANG Guo-lu (1. School of Agriculture and Food Science, Zhejiang Forestry College, Lin＇an 311300, Zhejiang, China; 2. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China) |
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| Abstract: | To determine colchicine＇s best concentration and treating time for inducing polyploidy in colorful calla lily, test materials with yellow cultivar of bulbs from Zantedeschia elliottiana ＇Black Magic＇ , were induced in vitro to form small buds from callus. Then, the small buds were soaked in the chemical reagent colchicine of 0.2, 0.5 and 1.0 g ·L^-1 for 24, 48 or 72 hours with 30 replications to induce polyploidy. Then, count the number of chromosome, and compare morphologic difference between the polyploidy and the diploid. Results showed that soaking small buds in 0.5 g·L^-1 of colchicine for 48 hours could successfully induce tetraploid with an induction rate of 13.3%. The tetraploid were stronger and had thicker and larger leaves, larger guard cells, more chloroplasts, and less stoma density than the diploid. The tetraploid would mend the pace of new breed varieties. Ch, 3 fig. 3 tab. 18 ref.] |
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| Keywords: | horticulture Zantedeschia elliottiana tissue culture colchicine polyploidy induction |
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