| 铜鱼、圆口铜鱼和长鳍吻鮈外周血细胞显微观察 |
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| 引用本文： | 赵海鹏,王志坚,张富生,张耀光.铜鱼、圆口铜鱼和长鳍吻鮈外周血细胞显微观察[J].安徽农业科学,2010,38(30):16964-16966,16990. |
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| 作者姓名： | 赵海鹏 王志坚 张富生 张耀光 |
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| 作者单位： | [1]西南大学生命科学学院,重庆400715 [2]淡水鱼类资源与生殖发育教育部重点实验室,重庆400715 [3]河南大学生命科学学院,河南开封475004 [4]濮阳外国语学校,河南濮阳457000 |
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| 摘 要： | 目的]为鱼类血液学研究积累资料，同时为进一步驯养提供借鉴。方法]对29尾铜鱼、27尾圆口铜鱼和17尾长鳍吻的尾静脉取血，常规方法每尾制作血涂片2-5张，Gimesa和Wright染液联合染色，Nikon—DXM1200数码显微摄影系统观察和拍照，ImageProplus软件随机测量各类血细胞。结果]观察到红细胞、嗜中性粒细胞、单核细胞、淋巴细胞和血栓细胞5种血细胞；红细胞有直接分裂现象；成红细胞有2种类型，单核细胞呈不规则球形，淋巴细胞有“大”、“小”2型。结论]共有5种血细胞，无嗜碱和嗜酸性粒细胞；“大”、“小”2型淋巴细胞并无定量区别；各种血细胞单核细胞最少，血栓细胞最多。
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| 关 键 词： | 铜鱼 圆口铜鱼 长鳍吻鮈 血细胞 |
Microscopic Structures of Peripheral Hematocytes in Coreius heterodon,Coreius guickenoti and Rhinogobio ventralis |
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| Institution: | ZHAO Hai-peng et al ( School of Life Science, Southwest University, Chongqing 400715 ) |
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| Abstract: | Objective]The aim was to collect the material of fish haematology and provide the reference for its artificial raising.Method]Blood was collected from 29 Coreius heterodon,27 Coreius guickenoti and 17 Rhinogobio ventralis.Blood smears were made and stained with Wright and Giemsa dying liquid.The morphological characteristics of the blood cells were evaluated using Nikon-DXM1200 digital microphotography and tested with Image Pro plus.Result]Erythrocytes,neutropils,monocytes,lymphocytes,and thrombocytes were observed.Erythrocytes were divided directly.There were two types of erythroblast (immature erythrocyte).The lympohcytes were irregular spherical form and two classes of lymphocytes(large and small) were seen.Conclusion]There were 5 types of peripheral blood cells of the fish,and no quantitative distinction was made between large and small lymphocytes.Monocytes were the least prevalent of the white blood cells in all species.Thrombocyte shapes were variable being either elongate,oval,spiked,spindle or some unable-describe forms,and they were most prevalent leucocyte type in all species.The basophils and eosinophils were absent. |
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| Keywords: | Coreius heterodon Coreius guickenoti Rhinogobio ventralis Erythrocyte |
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