| 丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究 |
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| 引用本文： | 毛碧飞,袁丽霞,陈祥松,孙立洁,朱微微,吴金勇,刘伟伟.丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究[J].安徽农业科学,2017,45(5). |
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| 作者姓名： | 毛碧飞 袁丽霞 陈祥松 孙立洁 朱微微 吴金勇 刘伟伟 |
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| 作者单位： | 中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031;中国科学院合肥物质科学研究院等离子体物理研究所,安徽合肥,230031 |
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| 基金项目： | 合肥物质科学技术中心方向项目培育基金，安徽省淮南市科技计划项目，中国科学院合肥分院等离子体物理研究所科学基金 |
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| 摘 要： | 目的]通过废弃资源的再利用研究可替代的新型生物燃料——丁醇的发酵培养基。方法]对菌株Clostridium acetobutylicum CGMCC 1.0134进行紫外诱变和磁场与Fe~(2+)共同诱变,获得1株丁醇产量高和稳定性好的优良突变株Clostridium acetobutylicum UM-80,采用不同的废弃原料考察该突变菌株的发酵性能,并筛选出合适的性价比高的培养基。结果]突变株UM-80发酵产丁醇和总溶剂(丙酮、丁醇、乙醇)分别为9.04、17.95 g/L,较原始菌株分别提高了5.12%、4.12%。单独的蕨根是较好的丁醇发酵原料,当蕨根醪质量分数为15%时发酵液中丁醇浓度最高为5.50 g/L。当高山被孢霉与蕨根共同发酵时发酵液中丁醇最高产量为6.50 g/L。结论]蕨根与高山被孢霉的复合培养基是较好的生物丁醇发酵培养基。
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| 关 键 词： | 丙酮丁醇梭菌 复合诱变 废弃物原料 丁醇 |
Study on Complex Mutagenesis of Clostridium acetobutylicum and Fermentation of Butanol from Waste Materials |
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| MAO Bi-fei,YUAN Li-xia,CHEN Xiang-song,SUN Li-jie,ZHU Wei-wei,WU Jin-yong,LIU Wei-wei.Study on Complex Mutagenesis of Clostridium acetobutylicum and Fermentation of Butanol from Waste Materials[J].Journal of Anhui Agricultural Sciences,2017,45(5). |
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| Authors: | MAO Bi-fei YUAN Li-xia CHEN Xiang-song SUN Li-jie ZHU Wei-wei WU Jin-yong LIU Wei-wei |
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| Abstract: | Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources.Method] Clostridium acetobutylicum UM-80,a mutant with high butanol yield and good stability,was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe2+ by Clostridium acetobutylicum CGMCC 1.0134.Then the different fermentation materials were used to study the fermentation performance of the mutant strains,and the suitable medium with high cost performance was found out.Result] The yield of butanol and total solvent (acetone,butanol,ethanol) were 9.04 and 17.95 g/L,respectively,which were 5.12％ and 4.12％ higher than those of the original strain.The fern root was the best raw material for the fermentation of butanol;when the mass fraction of fern root was 15％,the concentration of butanol in fermentation broth was 5.50 g/L.When the co-fermentation of Mortierella alpina and fern root,the highest yield was 6.50 g/L.Conclusion] The co-fermentation of Mortierella alpina and fem root is better fermentation medium for butanol. |
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| Keywords: | Clostridium acetobutylicum Compound mutagenesis Waste raw material Butanol |
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