| 水稻白叶枯病菌fkbM基因对其运动性的影响 |
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| 引用本文： | 伍甜.水稻白叶枯病菌fkbM基因对其运动性的影响[J].安徽农业科学,2017,45(5). |
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| 作者姓名： | 伍甜 |
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| 作者单位： | 佛山市高明区明城镇农业技术推广中心,广东佛山,528518 |
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| 摘 要： | 目的]研究水稻白叶枯病菌fkb M基因对其运动性的影响。方法]采用同源置换方法对水稻白叶枯病菌野生型菌株PXO99A的fkbM基因进行基因敲除,构建突变菌株ΔfkbM_(Xoo),同时构建互补菌株ΔfkbM_(Xoo)(C)。通过运动性试验,确定fkbM基因对运动性的影响。结果]与野生型菌株PXO99~A相比,ΔfkbM_(Xoo)突变体运动性明显减弱,而互补菌株ΔfkbM_(Xoo)(C)能基本恢复水稻白叶枯病菌的运动性。结论]水稻白叶枯病菌fkbM基因突变能减弱水稻白叶枯病菌的运动能力。
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| 关 键 词： | fkbMxoo 运动性 水稻白叶枯病菌 |
Effect of the fkbM Gene of Xanthomonas oryzae pv.oryzae on Motility |
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| WU Tian.Effect of the fkbM Gene of Xanthomonas oryzae pv.oryzae on Motility[J].Journal of Anhui Agricultural Sciences,2017,45(5). |
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| Authors: | WU Tian |
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| Abstract: | Objective] To study the effect of thefkbM gene of Xanthomonas oryzae pv.oryzae on motility.Method] A deletion mutant offkbM was generated by homologous recombination △fkbMxoo.To construct a complementary strain △fkbMXoo (C).Using motiliy test experiment to confirm the effect of thefkbM gene on motility.Result] Compared with wild type strain PXO99A,the mutant △fkbMXoo displayed reduced motility.In complementary strain △fkbMXoo (C),changed phenotypes were restored to certain degree.Conclusion] ThefkbM gene mutation of X.oryzae could reduce motility function of X.oryzae. |
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| Keywords: | fkbMXoo Motility Xanthomonas oryzae pv oryzae |
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