| 软枣猕猴桃原生质体酶解液配方筛选 |
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| 引用本文： | 付鹏跃,刘德江,申健,吕明医,梁雪莹,董实伟,程海涛.软枣猕猴桃原生质体酶解液配方筛选[J].安徽农业科学,2017,45(5). |
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| 作者姓名： | 付鹏跃 刘德江 申健 吕明医 梁雪莹 董实伟 程海涛 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007 |
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| 基金项目： | 黑龙江省大学生创新项目 |
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| 摘 要： | 目的]筛选软枣猕猴桃原生质体最佳酶解液配方。方法]以4种配方酶解液在相同条件下处理等量软枣猕猴桃叶片,比较最后收集的纯化原生质体量,筛选最佳酶解液配方。结果]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)和Ⅳ(1.0%纤维素酶、0.5%果胶酶)处理酶解效果要明显优于配方Ⅰ(2.0%纤维素酶、0.5%离析酶)、Ⅱ(2.0%纤维素酶、0.3%果胶酶)。结论]配方Ⅲ(1.0%纤维素酶、0.5%离析酶、0.3%果胶酶)为软枣猕猴桃原生质体最佳酶解液配方。
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| 关 键 词： | 软枣猕猴桃 原生质体 酶解 配方筛选 |
Screening of Enzymatic Hydrolysate Formula of Actinidia arguta Protoplast |
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| FU Peng-yue,LIU De-jiang,SHEN Jian,LV Ming-yi,LIANG Xue-ying,DONG Shi-wei,CHENG Hai-tao.Screening of Enzymatic Hydrolysate Formula of Actinidia arguta Protoplast[J].Journal of Anhui Agricultural Sciences,2017,45(5). |
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| Authors: | FU Peng-yue LIU De-jiang SHEN Jian LV Ming-yi LIANG Xue-ying DONG Shi-wei CHENG Hai-tao |
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| Abstract: | Objective] To screen the best enzymatic hydrolysate formula of Actinidia arguta protoplast.Method] The leaves of A.arguta were treated with four enzymatic hydrolysate formula under the same conditions.The purified protoplast was collected and compared to screen the optimal enzymatic hydrolysate formula.Result] The amount of protoplast collected by the solution of formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) and formula Ⅳ (1.0％ cellulase,0.5％ pectinase)were significantly higher than the solution of formula Ⅰ (2.0％ cellulase,0.5％ segregation enzyme) and formula Ⅱ (2.0％ cellulase,0.3％ pectinase).Conclusion] Formula Ⅲ (1.0％ cellulase,0.5％ segregation enzyme and 0.3％ pectinase) is the best enzymatic hydrolysate formula for A.arguta protoplast. |
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| Keywords: | Actinidia arguta Protoplast Enzymatic hydrolysis Formula screening |
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