| 猪肌生成抑制素基因的克隆和序列测定 |
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| 引用本文： | 欧阳红生,孙燕,张永亮,李慎涛,张锐,廖晓萍,张玉静,赵建军,赵凤云.猪肌生成抑制素基因的克隆和序列测定[J].中国兽医学报,2001,21(5):479-481. |
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| 作者姓名： | 欧阳红生 孙燕 张永亮 李慎涛 张锐 廖晓萍 张玉静 赵建军 赵凤云 |
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| 作者单位： | 解放军军需大学基础部,吉林长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目(30070563):军队医药卫生基金资助项目(98Q079) |
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| 摘 要： | 根据文献报道的猪肌生成抑制素基因cDNA序列，分别从第1、第2和第3外显子中设计引物，以大约克猪染色体DNA为模板，分段扩增出含内含子和外显子的序列。将所得各序列回收、克隆到pGEM-easyT载体中，然后进行序列测定。合并所有测得的猪肌生成抑制素因序列，共获得6kb连续序列。分析结果表明，猪肌生成抑制素基因第1内含子长1809bp，第2外显子长374bp，第2内含子长1978bp。所测得的外显子序列与已发表的cDNA序列同源分析比较，没有出现引起氨基酸改变的突变。表明瘦肉型猪（大约克猪）肌生成抑制素基因仍很完整，可通过改变基因结构等降低肌生成抑制素活性的方法提高瘦肉率。
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| 关 键 词： | 猪 肌生成抑制素 基因 克隆 序列 |
| 文章编号： | 1005-4545(2001)05-0479-03 |
| 修稿时间： | 2000年9月6日 |
Cloning and Sequence Analysis of Porcine Myostatin Gene |
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| OUYANG Hong-sheng,SUN Yan,ZHANG Yong-liang,LI Shen-tao,ZHANG Rui,L IAO Xiao-ping,ZHANG Yu-jing,ZHAO Jian-jun,ZHAO Fang-yun.Cloning and Sequence Analysis of Porcine Myostatin Gene[J].Chinese Journal of Veterinary Science,2001,21(5):479-481. |
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| Authors: | OUYANG Hong-sheng SUN Yan ZHANG Yong-liang LI Shen-tao ZHANG Rui L IAO Xiao-ping ZHANG Yu-jing ZHAO Jian-jun ZHAO Fang-yun |
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| Abstract: | Ten oligonucleotide primers were designed and synthesized according to the nucleotide sequence of porcine myostatin c DNA. Using the primers,the whole gene of porcine myostatin was amplified by PCR from porcine DNA which extracted from pig blood. Four fragments ( the upstream flanking region-the exon 1 ,the intron 1 -exon 2 ,the in- tron 2 -exon 3,and the exon 3) ,prepared from PCR products,were cloned into the p GEM-T Easy Vector( Promega) . The nucleotide sequences of all four fragments from the recombinant plasmid were determined. The 6 kb nucleotide se- quence of the whole porcine myostatin gene was obtained. The result showed that the intron 1 and the intron 2 of porcine myostatin gene must be 1 80 9bp and 1 978bp,rsepectively. |
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| Keywords: | porcine myostatin gene cloning sequence |
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