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大豆品种资源抗灰斑病7号生理小种优异等位变异的发掘
引用本文:蒋红娇,赵慧艳,林春雨,梁晓宇,马淑梅,王洋.大豆品种资源抗灰斑病7号生理小种优异等位变异的发掘[J].中国农学通报,2020,36(31):97-102.
作者姓名:蒋红娇  赵慧艳  林春雨  梁晓宇  马淑梅  王洋
作者单位:黑龙江大学现代农业与生态环境学院,哈尔滨 150080
基金项目:农业农村部作物种质资源保护“主要作物抗病虫、抗逆和品质性状鉴定评价”(2018NWB036-12)
摘    要:本文旨在发掘大豆品种资源中抗灰斑病7号生理小种优异等位变异,为培育抗灰斑病品种提供遗传信息和载体材料。以202份大豆品种为试验材料,鉴定材料抗灰斑病7号生理小种的抗病性。利用SSR标记进行全基因组扫描,采用TASSEL 3.0软件的GLM模型和MLM模型对大豆品种的灰斑病抗性与标记进行关联分析。结果表明,2种模型共同检测到12个与灰斑病7号生理小种抗性显著关联的位点,各标记对表型变异的解释率为2.00%~15.47%,共同检测到增效作用的等位变异共有20个,其中增效表型效应值最大的等位变异是Satt549-263(18.88),载体材料为‘合丰29’;其次为Satt372-291(17.92),载体材料为‘合丰34’。发掘到的等位变异信息和载体材料为培育抗灰斑病品种亲本选配和后代等位条带辅助选择提供了依据。

关 键 词:大豆  灰斑病  7号生理小种  等位变异  连锁不平衡  
收稿时间:2020-04-20

Soybean Variety Resource Resistant to Cercospora sojina Race 7: Mining of Elite Alleles
Jiang Hongjiao,Zhao Huiyan,Lin Chunyu,Liang Xiaoyu,Ma Shumei,Wang Yang.Soybean Variety Resource Resistant to Cercospora sojina Race 7: Mining of Elite Alleles[J].Chinese Agricultural Science Bulletin,2020,36(31):97-102.
Authors:Jiang Hongjiao  Zhao Huiyan  Lin Chunyu  Liang Xiaoyu  Ma Shumei  Wang Yang
Institution:Department of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
Abstract:The objective is to explore the elite allele of soybean variety resource resistant to Cercospora sojina race 7, and provide genetic information and carrier materials for breeding soybean varieties resistant to C. sojina. In this study, 202 commercial soybean varieties were used as the experimental materials, and the disease resistance to race 7 of C. sojina was identified. The whole genome scanning was used by SSR markers. Association analysis for soybean varieties’ resistance to C. sojina was performed by GLM and MLM model. The results showed that a total of 12 loci were detected by GLM and MLM model, which were significantly associated with the resistance to C. sojina race 7. The rate of explanation on the phenotype of related marker ranged from 2.00% to 15.47%. 20 positive alleles were revealed, among which Satt549-263(18.88) was the largest positive allele in the phenotypic effect value of C. sojina, and ‘Hefeng 29’ was the carrier material. The second one was Satt372-291(17.92), and the carrier material was ‘Hefeng 34’. The information of elite alleles and carrier materials could provide evidence for cross design for pyramiding elite alleles and marker-assisted selection in breeding of varieties resistant to C. sojina.
Keywords:soybean  Cercospera sojina  Race 7  elite alleles  linkage disequilibrium  
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