| 不同温度和pH对恩诺沙星及其代谢产物环丙沙星在渔业下层水体中消除规律的影响 |
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| 引用本文： | 黄铢玉,方龙香,宋超,Mussa Ramadhan Said,杨光,李天,陈家长.不同温度和pH对恩诺沙星及其代谢产物环丙沙星在渔业下层水体中消除规律的影响[J].中国农学通报,2020,36(21):132-139. |
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| 作者姓名： | 黄铢玉 方龙香 宋超 Mussa Ramadhan Said 杨光 李天 陈家长 |
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| 作者单位： | 1. 南京农业大学无锡渔业学院,江苏无锡 214081;2. 中国水产科学研究院淡水渔业研究中心,江苏无锡 214081;3. 农业农村部水产品质量安全环境因子风险评估实验室（无锡）,江苏无锡 214081;4. 农业农村部水产品质量安全控制重点实验室,北京 100141;5. 中国水产科学研究院渔业工程研究所,北京 100141 |
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| 基金项目： | 国家重点研发计划“水产品全链条关键危害物的迁移转化规律与安全防控技术研究”(2017YFC1600704);中央级科研院所基本科研业务费“水产养殖抗生素减量与替代的精准化技术研究”(2019JBFZ04);国家水产品质量安全风险评估项目“大闸蟹中环境污染物残留风险评估”(GJFP2019003) |
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| 摘 要： | 抗生素进入水体易堆积在水体下层或者底泥中,对水生生物造成不良影响,因此本实验旨在探究恩诺沙星在渔业下层水体中的消除规律,以及代谢产物环丙沙星的生成情况。实验选取了渔业水环境中常见的环境因素（温度和pH）,利用液相色谱-串联质谱法,通过正交实验研究这两个环境因素对恩诺沙星(ENR)在水体中降解的影响,同时检测其代谢产物环丙沙星(CIP)的生成及变化情况。实验结果表明,9个处理组中ENR的半衰期变化范围为13~32天,消除率范围为44%~80%。根方差分析可知,pH变化对恩诺沙星的降解有显著影响,而温度变化对其影响不显著,在温度为25℃和pH 5的条件下,恩诺沙星的消除率最高。此外,在实验过程中检测到环丙沙星的产生,其在实验开始的第7天,在pH 5和温度为25℃的条件下浓度达到最高(10.8 ng/mL),之后逐渐降低,在第26天时浓度降低到0~0.16 ng/mL之间。本研究明确了环境因素对恩诺沙星降解的影响,为减少养殖环境中残留的恩诺沙星提供一定的理论指导。
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| 关 键 词： | 恩诺沙星 环丙沙星 下层水体 环境因素 消除规律 |
| 收稿时间： | 2020-01-08 |
Temperature and pH: Effect on the Elimination Rule of Enrofloxacin and Its Metabolite Ciprofloxacin in Fishery Lower Water Bodies |
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| Huang Zhuyu,Fang Longxiang,Song Chao,Mussa Ramadhan Said,Yang Guang,Li Tian,Chen Jiazhang.Temperature and pH: Effect on the Elimination Rule of Enrofloxacin and Its Metabolite Ciprofloxacin in Fishery Lower Water Bodies[J].Chinese Agricultural Science Bulletin,2020,36(21):132-139. |
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| Authors: | Huang Zhuyu Fang Longxiang Song Chao Mussa Ramadhan Said Yang Guang Li Tian Chen Jiazhang |
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| Abstract: | The aim is to investigate the elimination patterns of enrofloxacin in the lower water column of the fishery and the production of the metabolite ciprofloxacin, since antibiotics entering the water column are prone to accumulate in the lower water column or in the substrate sediment, and cause adverse effects on aquatic life. Common environmental factors (temperature and pH) in the fishery water were selected for the experiment, and effects of these two environmental factors on the degradation of enrofloxacin (ENR) in aqueous humor were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with orthogonal experiments, and the production and changes of the metabolite ciprofloxacin (CIP) were detected. The experimental results showed that the half-life variation of ENR in the nine treatment groups ranged from 13 to 32 d and the elimination rate ranged from 44% to 80%. Root ANOVA knots showed that pH changes had a significant effect on the degradation of enrofloxacin, while temperature changes did not have a significant effect, with the highest elimination rate of enrofloxacin at 25°C and pH 5. In addition, the production of ciprofloxacin was detected during the experiment, which reached the highest concentration (10.8 ng/mL) at pH 5 and 25°C on day 7 from the beginning of the experiment, and then gradually decreased to the concentration 0-0.16 ng/mL on day 26. This study clarifies the influence of environmental factors on enrofloxacin degradation and provides some theoretical guidance for reducing enrofloxacin residues in aquaculture environment. |
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| Keywords: | enrofloxacin ciprofloxacin lower water body environmental factors eliminate rule |
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