| 表达猪传染性胃肠炎病毒S蛋白抗原表位乳酸菌对实验小鼠免疫功能的影响 |
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| 引用本文： | 杜颖卉,张艺凡,王书全.表达猪传染性胃肠炎病毒S蛋白抗原表位乳酸菌对实验小鼠免疫功能的影响[J].中国农学通报,2020,36(23):130-133. |
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| 作者姓名： | 杜颖卉 张艺凡 王书全 |
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| 作者单位： | 锦州医科大学畜牧兽医学院,辽宁锦州 121001 |
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| 基金项目： | 辽宁省自然基金项目“PEDV/TGEV多抗原表位与SEA基因共表达重组沙门氏菌构建及免疫原性研究”(201602320);锦州医科大学大学生创新创业项目“TGEV多抗原表位与SEA基因共表达重组沙门氏菌的构建与免疫原性研究”(201610160025) |
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| 摘 要： | 为了探讨表达猪传染性胃肠炎病毒(TGEV)S蛋白抗原表位乳酸乳球菌对小鼠安全性及免疫功能,以口服的方式给实验小鼠免疫重组乳酸菌,观察小鼠的精神状态,采用ELISA试剂盒方法检测免疫指标。结果表明：重组乳酸菌对小鼠的精神状态及体重均无影响,能显著提高小鼠血清中IgG和粪便sIgA的含量。与对照组相比,免疫后1周、2周、5周的血清中IgG的含量差异显著(P<0.05),3周、4周差异极显著(P<0.01),表明能显著提高小鼠体液免疫水平;能显著提高外周血中T淋巴细胞增殖情况,与对照组相比,差异极显著(P<0.01),表明能显著提高小鼠细胞免疫水平。表达TGEV S蛋白抗原表位乳酸菌能显著提高小鼠体液免疫和细胞免疫水平,具有良好的免疫原性,且安全可靠。
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| 关 键 词： | TGEV 重组乳酸乳球菌 免疫功能 口服免疫 实验小鼠 |
| 收稿时间： | 2019-06-28 |
Effect of Surface-displayed TGEV-S Epitopes in Lactococcus Lactis on the Immune Function of Experimental Mice |
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| Du Yinghui,Zhang Yifan,Wang Shuquan.Effect of Surface-displayed TGEV-S Epitopes in Lactococcus Lactis on the Immune Function of Experimental Mice[J].Chinese Agricultural Science Bulletin,2020,36(23):130-133. |
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| Authors: | Du Yinghui Zhang Yifan Wang Shuquan |
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| Institution: | College of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou Liaoning 121001 |
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| Abstract: | To investigate the safety and immune function of lactococcus lactis expressing S protein epitope of porcine transmissible gastroenteritis virus (TGEV) in mice, the experimental mice were orally immunized with recombinant lactococcus lactis, the mental state were observed and the immune indexes were determined using an ELISA kit according to the instructions. The results showed that recombinant lactococcus lactis had no effect on the mental state and body weight of mice, and increased serum IgG and fecal sIgA content significantly. Compared with the control group, the difference was significant in one week, two weeks and five weeks after immunization, extremely significant in three weeks and four weeks after immunization, indicating that Lactococcus lactis could improve humoral immunity level of mice obviously. The recombinant lactococcus lactis could obviously enhance T lymphocyte proliferation in peripheral blood, compared with the control group, the difference was very significant, which indicated that the recombinant lactococcus lactis could improve cellular immunity level of mice. Lactococcus lactis expressing the surface-displayed TGEV-S epitopes could obviously improve humoral immunity and cellular immunity level, has good immunogenicity and is safe and reliable. |
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| Keywords: | TGEV recombinant Lactococcus lactis immune function orally immunization experimental mice |
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