| 基于简化基因组的甜叶菊分子标记开发 |
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| 引用本文： | 孙雨茜,倪万潮,杜平,崔晓霞,郭书巧,蒋璐.基于简化基因组的甜叶菊分子标记开发[J].中国农学通报,2020,36(27):111-117. |
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| 作者姓名： | 孙雨茜 倪万潮 杜平 崔晓霞 郭书巧 蒋璐 |
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| 作者单位： | 1.江苏省农业科学院中心实验室,南京 210014;2.江苏省农业科学院经济作物所/江苏省生物学重点实验室,南京 210014 |
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| 基金项目： | 江苏省农业科学院院基金项目“基于KASP技术的甜叶菊真实性鉴定方法”(6111692) |
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| 摘 要： | 本研究旨在筛选和开发具有多态性的甜叶菊基因组分子标记。选取4个甜叶菊品种为材料,利用简化基因组测序技术(RAD-seq)开发SSR和SNP标记。开发并验证了149对多态性丰富、重复性好、特异性高的简单重复序列(SSR)和4组功能性的SNP标记,开发的多态性基因组分子标记中包含了甜叶菊糖苷合成途径关键基因的新标记StKASPSNP-7。验证了基于KASP技术开发甜叶菊功能SNP标记的可行性,为甜叶菊遗传图谱构建、分子鉴定奠定了基础。本研究开发的SSR和SNP标记可应用于甜叶菊的遗传图谱分析、品种真实性的高效快速鉴定以及高糖苷含量新品种的开发。
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| 关 键 词： | 甜叶菊 简化基因组测序(RAD-seq) 简单重复序列(SSR) 单核苷酸多态性(SNP) 竞争性等位基因特异性PCR(KASP) |
| 收稿时间： | 2019-08-22 |
Development of Molecular Markers in Stevia rebaudiana Based on Reduced-Representation Genome Sequencing |
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| Sun Yuxi,Ni Wanchao,Du Ping,Cui Xiaoxia,Guo Shuqiao,Jiang Lu.Development of Molecular Markers in Stevia rebaudiana Based on Reduced-Representation Genome Sequencing[J].Chinese Agricultural Science Bulletin,2020,36(27):111-117. |
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| Authors: | Sun Yuxi Ni Wanchao Du Ping Cui Xiaoxia Guo Shuqiao Jiang Lu |
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| Institution: | 1.Central Laboratory, Jiangsu Academy of Agricultural Sciences, Nanjing 210014;2.Key Laboratory for Jiangsu Province Agrobiology/Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014 |
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| Abstract: | The aim is to select and develop polymorphic molecular markers of Stevia rebaudiana genome. 4 Stevia rebaudiana varieties were used as materials, SSR markers and SNP markers were developed with RAD-seq. 149 pairs of SSR with rich polymorphism, high repeatability and specificity, and 4 functional SNP markers were developed and identified. These new genome molecule markers included new markers StKASPSNP-7 of key gene in Stevia rebaudiana glycoside synthesis pathway. The feasibility of developing SNP markers of Stevia rebaudiana based on KASP technology was verified, which laid a foundation for constructing genetic map and molecular identification of Stevia rebaudiana. SSR markers and KASP markers developed in the study could be used to analyze the genetic map of Stevia rebaudiana, identify its variety authenticity with high efficiency and speed, and develop new varieties with high glycoside content. |
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| Keywords: | Stevia rebaudiana RAD-seq SSR SNP KASP |
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