| 四川省无花果疫霉病病原鉴定 |
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| 引用本文： | 翟敏,鲜贇曦,黄小琴,张蕾,周西全,刘勇,杨潇湘.四川省无花果疫霉病病原鉴定[J].中国农学通报,2020,36(17):123-128. |
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| 作者姓名： | 翟敏 鲜贇曦 黄小琴 张蕾 周西全 刘勇 杨潇湘 |
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| 作者单位： | 1. 四川省农业科学院植物保护研究所,成都 610066;2. 农业农村部西南作物有害生物综合治理重点实验室,成都 610066 |
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| 基金项目： | 四川省农业科学院优秀论文基金(2017LWJJ-004) |
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| 摘 要： | 为明确四川省无花果爆发的疫霉病病原菌种类,对四川省无花果种植区的无花果疫霉病的发生进行调查,并采集发病果实。采用组织分离法对无花果疫霉病的病原菌进行分离培养,并通过形态与分子鉴定相结合对该病原菌进行鉴定。结果表明,病原菌菌丝为白色;卵孢子球形;孢子囊椭圆形或卵形,顶端有乳突。分子生物学鉴定结果显示：四川省无花果疫霉病病原菌与棕榈疫霉(Phytophthora palmivora)序列同源性高达99%。根据形态学和分子鉴定,将四川省无花果疫霉病病原菌鉴定为棕榈疫霉(Phytophthora palmivora)。
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| 关 键 词： | 无花果 疫霉病 病原真菌 分离鉴定 棕榈疫霉 |
| 收稿时间： | 2020-01-16 |
Identification of the Pathogen of Ficus carica Epidemic Disease in Sichuan |
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| Zhai Min,Xian Yunxi,Huang Xiaoqin,Zhang Lei,Zhou Xiquan,Liu Yong,Yang Xiaoxiang.Identification of the Pathogen of Ficus carica Epidemic Disease in Sichuan[J].Chinese Agricultural Science Bulletin,2020,36(17):123-128. |
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| Authors: | Zhai Min Xian Yunxi Huang Xiaoqin Zhang Lei Zhou Xiquan Liu Yong Yang Xiaoxiang |
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| Institution: | 1. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066;2. Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops of Ministry of Agriculture and Rural Affairs, Chengdu 610066 |
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| Abstract: | To identify the pathogen species of Ficus carica epidemic disease outbreak in Sichuan Province, the occurrence of F. carica epidemic disease in F. carica planting areas in Sichuan was investigated, and the diseased fruits were collected. The pathogen was isolated and cultured by tissue isolation method, and was identified by combining morphology and molecular identification. The results showed that the pathogen mycelium was white, the oospores were spherical, and the sporangiums were oval or ovoid, with papillae at the top. The sequence analysis revealed 99% sequence identities with Phytophthora palmivora. According to morphology and molecular identification, the pathogen of F. carica epidemic disease in Sichuan Province was identified as P. palmivora. |
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| Keywords: | Ficus carica phytophthora blight pathogenic fungi isolation and identification Phytophthora palmivora |
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